Koura A N, Liu W, Kitadai Y, Singh R K, Radinsky R, Ellis L M
Department of Surgical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Cancer Res. 1996 Sep 1;56(17):3891-4.
To determine the effect of cell density on vascular endothelial growth factor (VEGF) expression and the mechanism of this effect, four human colon cancer cell lines were grown as sparse or confluent monolayers or as spheroids. VEGF mRNA increased > 2-fold in cells grown as confluent monolayers or spheroids compared with cells grown as sparse monolayers. Semiquantitative reverse transcription-PCR demonstrated a 2-fold increase in the larger VEGF mRNA isoform (189 bp) in confluent cells. Sparse cells grown in conditioned medium from confluent cells demonstrated a > 2-fold increase in VEGF mRNA. These data suggest that VEGF expression may be regulated by an unidentified soluble factor.
为了确定细胞密度对血管内皮生长因子（VEGF）表达的影响及其作用机制，将四种人结肠癌细胞系培养成稀疏或汇合的单层细胞或球体。与稀疏单层培养的细胞相比，汇合单层培养或球体培养的细胞中VEGF mRNA增加了2倍以上。半定量逆转录 - PCR显示汇合细胞中较大的VEGF mRNA异构体（189 bp）增加了2倍。在来自汇合细胞的条件培养基中培养的稀疏细胞中，VEGF mRNA增加了2倍以上。这些数据表明VEGF表达可能受一种未鉴定的可溶性因子调控。
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