Zhu Jianwei, Yu Baoming, Ji Yubao, Zheng Minhua, Li Donghua
Department of General Surgery, RuiJin Hospital Affiliated to Shanghai Second Medical University, Shanghai 200025, China.
Zhonghua Yi Xue Za Zhi. 2002 Feb 25;82(4):271-4.
To clarify the possible effect of reactive oxygen species such as hydrogen peroxide on progression of human colorectal cancer.
Human colon carcinoma cell lines, L174T and HCT8, were treated with low concentration of hydrogen peroxide (10(-5) mol/L, 10(-7) mol/L and 10(-9) mol/L, possessed no effect on cancer cell growth) for 24 hours before being co-cultured with human endothelial cell line ECV-304. The migration of ECV-304 induced by cancer cells was calculated and the expression of vascular endothelial growth factor (VEGF) in cancer cells was determined using RT-PCR and ELISA. Dactinomycin (1.5 microg/ml) was applied to block the transcription of cancer cell so as to observe the effect site of cell. Finally the activity of NF-KappaB of cancer cells was estimated using laser scanning cytometry.
Exogenous hydrogen peroxide of low concentration promoted the migration of endothelial cell induced by colon cancer cell. When the concentration of H(2)O(2) was 10(-5) mol/L, the average number of migration of endothelial cell induced by LS174T was 202.6 +/- 70.2, higher than the value of 154.6 +/- 37.9 when no H(2)O(2) was given (t = 3.4751, P = 0.0083). When the concentration of H(2)O(2) was 10(-5) mol/L, the average number of migration of endothelial cell induced by HCT8 was 145.4 +/- 65.3, higher than the value of 113 +/- 72.7 when no H(2)O(2) was given (t = 4.4183, P = 0.0084). The expression of vascular endothelial growth factor in cancer cells was enhanced to a certain degree, which could be blocked by Dactinomycin. And the half-time of VEGF mRNA was not prolonged by treatment with hydrogen peroxide. The activity of NF-KappaB of both colon carcinoma cell lines was at a higher level after being treated with hydrogen peroxide for 24 hours.
Hydrogen peroxide increases vascular endothelial growth factor expression in colon cancer cell, and it is possible that such reactive oxygen species as hydrogen peroxide facilitates development of colon cancer.
阐明过氧化氢等活性氧对人类结直肠癌进展的可能影响。
将人结肠癌细胞系L174T和HCT8用低浓度过氧化氢（10⁻⁵mol/L、10⁻⁷mol/L和10⁻⁹mol/L，对癌细胞生长无影响）处理24小时，然后与人内皮细胞系ECV - 304共培养。计算癌细胞诱导的ECV - 304的迁移情况，并使用逆转录聚合酶链反应（RT - PCR）和酶联免疫吸附测定（ELISA）法测定癌细胞中血管内皮生长因子（VEGF）的表达。应用放线菌素D（1.5μg/ml）阻断癌细胞转录，以观察细胞的作用位点。最后使用激光扫描细胞术评估癌细胞中核因子κB（NF - κB）的活性。
低浓度外源性过氧化氢促进结肠癌细胞诱导的内皮细胞迁移。当H₂O₂浓度为10⁻⁵mol/L时，LS174T诱导的内皮细胞平均迁移数为202.6±70.2，高于未给予H₂O₂时的154.6±37.9（t = 3.4751，P = 0.0083）。当H₂O₂浓度为10⁻⁵mol/L时，HCT8诱导的内皮细胞平均迁移数为145.4±65.3，高于未给予H₂O₂时的113±72.7（t = 4.4183，P = 0.0084）。癌细胞中血管内皮生长因子的表达有一定程度增强，这可被放线菌素D阻断。过氧化氢处理未延长VEGF mRNA的半衰期。两种结肠癌细胞系在经过氧化氢处理24小时后，NF - κB的活性均处于较高水平。
过氧化氢增加结肠癌细胞中血管内皮生长因子的表达，过氧化氢等活性氧可能促进结肠癌的发展。
