Abe S, Kumanishi T
Department of Molecular Neuropathology, Niigata University.
Rinsho Shinkeigaku. 1995 Dec;35(12):1473-5.
In this study, we examined the VDJ region of the immunoglobulin heavy chain (IgH) gene in intravascular malignant lymphomatosis (IML). The VDJ regions were amplified using the polymerase chain reaction (PCR) with consensus primers of the V and J regions of the IgH gene. Specimens from all the IML cases produced a monoclonal band. Specimens from metastatic carcinomas, brain tumors and normal tissues produced no monoclonal amplification. By cloning and sequencing the amplified VDJ regions, we have determined nucleotide sequences of rearranged regions of the IgH genes. In addition, we also examined tumor suppressor genes. The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis revealed no abnormal migration in exons 5 to 8 of the p53 gene, exon 2 of the p16 gene and exon 2 of the p21 gene. These findings suggested that mutation of p53, p16 and p21 genes is rarely related with the tumorigenesis of IML. The presence of Epstein-Barr virus (EBV) was also analyzed using PCR. EBV DNA was detected in one of five IML specimens. This result indicates that IML may be associated with EBV.
在本研究中，我们检测了血管内恶性淋巴瘤（IML）中免疫球蛋白重链（IgH）基因的VDJ区域。使用针对IgH基因V区和J区的共有引物，通过聚合酶链反应（PCR）扩增VDJ区域。所有IML病例的标本均产生单一条带。转移性癌、脑肿瘤和正常组织的标本未产生单克隆扩增。通过对扩增的VDJ区域进行克隆和测序，我们确定了IgH基因重排区域的核苷酸序列。此外，我们还检测了肿瘤抑制基因。聚合酶链反应-单链构象多态性（PCR-SSCP）分析显示，p53基因外显子5至8、p16基因外显子2和p21基因外显子2均无异常迁移。这些发现表明，p53、p16和p21基因的突变与IML的肿瘤发生很少相关。还使用PCR分析了EB病毒（EBV）的存在情况。在5例IML标本中的1例中检测到EBV DNA。这一结果表明IML可能与EBV有关。
