Mizumoto Y
Department of Obstetrics and Gynecology, National Defense Medical College, Saitama.
Nihon Sanka Fujinka Gakkai Zasshi. 1996 Jun;48(6):379-85.
The purpose of this study was to clarify the relationship between NK activity and TG-beta in the immune system in endometriosis. We investigated (1) the changes in the NK activity and concentration of TGF-beta in human peritoneal fluid (HPF), and (2) the effects of HPF and TGF-beta on the development of early mice embryos. In a rat model of experimental endometriosis, we observed the effects of tissue culture supernatants of peritoneum on NK activity in rat spleen cells, and obtained the following results. (1) NK activity of peripheral lymphocytes in healthy women was significantly suppressed in the presence of HPF of endometriosis. (2) The concentrations of TGF-beta was significantly higher in HPF of endometriosis than in HPF of healthy women. (3) Both HPF of endometriosis and TGF-beta significantly inhibited the development of early mice embryos. (4) The supernatants prepared from the intact peritoneum of the rat model showed marked inhibition of NK activity compared to control rats, although the peritoneum was obtained from a region distant from the implanted endometrium. These results suggest that ectopic endometrial tissues may cause a change in the cell-mediated immune system and subsequently exert an adverse effect on human reproduction.
本研究的目的是阐明子宫内膜异位症患者免疫系统中自然杀伤细胞（NK）活性与转化生长因子-β（TG-β）之间的关系。我们进行了以下研究：（1）检测人腹腔液（HPF）中NK活性及TG-β浓度的变化；（2）观察HPF及TG-β对小鼠早期胚胎发育的影响。在实验性子宫内膜异位症大鼠模型中，我们观察了腹膜组织培养上清液对大鼠脾细胞NK活性的影响，结果如下：（1）子宫内膜异位症患者的HPF可显著抑制健康女性外周淋巴细胞的NK活性；（2）子宫内膜异位症患者HPF中TG-β的浓度显著高于健康女性；（3）子宫内膜异位症患者的HPF及TG-β均能显著抑制小鼠早期胚胎的发育；（4）尽管取自远离植入子宫内膜区域的腹膜，但大鼠模型完整腹膜制备的上清液与对照大鼠相比，仍显示出对NK活性的显著抑制作用。这些结果表明，异位子宫内膜组织可能导致细胞介导的免疫系统发生变化，进而对人类生殖产生不利影响。
