Kohata Y
The 4th Department of Internal Medicine, Tokyo Medical College, Japan.
Nihon Shokakibyo Gakkai Zasshi. 1996 Jun;93(6):391-7.
Using mutant-allele-specific amplification procedure, patients with colorectal tumors were analyzed for K-ras point mutations in the stool and the tumor tissue. K-ras mutation of DNA purified from the stool was detected in 10 of 40 (25.0%) cancer patients, and in 3 of 10 (30.0%) adenoma patients. Otherwise, in the cases whose tumors contained the mutations, it was detected with the frequency of 71.4% in cancers, and 100% in adenomas. This frequency tended to decrease in the cancers of distal colon or small size, but there was no significant. This study suggested that stool analysis of genetic alterations would develop diagnostic method for colorectal cancer.
采用突变等位基因特异性扩增方法，对结直肠肿瘤患者的粪便和肿瘤组织进行K-ras点突变分析。在40例癌症患者中，有10例（25.0%）粪便中纯化的DNA检测到K-ras突变，10例腺瘤患者中有3例（30.0%）检测到。此外，在肿瘤含有突变的病例中，癌症患者的检测频率为71.4%，腺瘤患者为100%。该频率在远端结肠癌或小尺寸癌症中趋于降低，但无显著差异。本研究表明，对基因改变进行粪便分析将为结直肠癌开发诊断方法。
