Ghohestani R F, Nicolas J F, Rousselle P, Claudy A L
Department of Dermatology & Institute of Health and Medical Researches (INSERM), E. Herriot Hospital, Lyon, France.
J Invest Dermatol. 1996 Jul;107(1):136-9. doi: 10.1111/1523-1747.ep12298424.
This study describes the presence of antibodies in sera from patients with cicatricial pemphigoid specific for a 168-kDa antigen expressed by buccal mucosa. Six cicatricial pemphigoid sera unreactive, with epidermal or dermal proteins in immunoblot assay were tested on mucosal protein extracts. Four of these sera labeled a mucosal 168-kDa antigen (M168) under reducing conditions. An additional cicatricial pemphigoid serum with circulating antibodies to 180-kDa bullous pemphigoid antigen (BPAg2) also labeled M168. None of these cicatricial pemphigoid sera reacted with the alpha, beta, or gamma subunits of laminin-5. Nitrocellulose elution studies showed that the M168 antigen is a basement membrane antigen and labeled the epidermal side of salt-split skin. Immunoaffinity-purified anti-M168 antibodies did not bind to the 230-kDa bullous pemphigoid antigen (BPAg1) or to the 180-kDa BPAg2. None of the control sera from healthy individuals or from bullous pemphigoid, pemphigus vulgaris, or pemphigus foliaceus patients reacted with Ml68. This study demonstrates the specificity of some cicatricial pemphigoid sera against a 168-kDa antigen that is different from the laminin-5 subunits and shares no epitopes with the antigens of bullous pemphigoid (BPAg1, BPAg2) or the epidermolysis bullosa acquisita.
本研究描述了瘢痕性类天疱疮患者血清中存在针对由颊黏膜表达的一种168 kDa抗原的抗体。对6份在免疫印迹试验中与表皮或真皮蛋白无反应的瘢痕性类天疱疮血清进行黏膜蛋白提取物检测。其中4份血清在还原条件下标记了一种黏膜168 kDa抗原（M168）。另一份含有针对180 kDa大疱性类天疱疮抗原（BPAg2）循环抗体的瘢痕性类天疱疮血清也标记了M168。这些瘢痕性类天疱疮血清均未与层粘连蛋白-5的α、β或γ亚基发生反应。硝酸纤维素洗脱研究表明，M168抗原是一种基底膜抗原，并标记了盐裂皮肤的表皮侧。免疫亲和纯化的抗M168抗体不与230 kDa大疱性类天疱疮抗原（BPAg1）或180 kDa BPAg2结合。来自健康个体或大疱性类天疱疮、寻常型天疱疮或落叶型天疱疮患者的对照血清均未与M168发生反应。本研究证明了一些瘢痕性类天疱疮血清针对一种168 kDa抗原的特异性，该抗原不同于层粘连蛋白-5亚基，且与大疱性类天疱疮（BPAg1、BPAg2）或获得性大疱性表皮松解症的抗原无共同表位。
