Thomson S A, Elliott S L, Sherritt M A, Sproat K W, Coupar B E, Scalzo A A, Forbes C A, Ladhams A M, Mo X Y, Tripp R A, Doherty P C, Moss D J, Suhrbier A
Queensland Institute of Medical Research, Brisbane, Australia.
J Immunol. 1996 Jul 15;157(2):822-6.
Development of epitope-based CD8 alpha beta CTL vaccines requires effective strategies for codelivery of large numbers of individual epitopes. We have designed an artificial "polyepitope" protein containing 10 contiguous minimal CTL epitopes, which were restricted by five MHC alleles and derived from five viruses, a parasite, and a tumor model. A recombinant vaccinia virus coding for this protein was capable of inducing MHC-restricted primary CTL responses to all 10 epitopes. Mice immunized with this recombinant vaccinia showed protection against murine cytomegalovirus, Sendai virus, and a tumor model. This simple generic approach to multiepitope delivery should find application in CTL-based vaccine design.
基于表位的CD8αβ细胞毒性T淋巴细胞（CTL）疫苗的开发需要有效的策略来共同递送大量单个表位。我们设计了一种人工“多表位”蛋白，它包含10个连续的最小CTL表位，这些表位受5种主要组织相容性复合体（MHC）等位基因限制，来源于5种病毒、1种寄生虫和1种肿瘤模型。编码这种蛋白的重组痘苗病毒能够诱导针对所有10个表位的MHC限制性初始CTL反应。用这种重组痘苗病毒免疫的小鼠对鼠巨细胞病毒、仙台病毒和一种肿瘤模型具有保护作用。这种简单的多表位递送通用方法应可应用于基于CTL的疫苗设计。
