Forestier M, Reichen J, Solioz M
Department of Clinical Pharmacology, University of Berne, Switzerland.
Biochem Biophys Res Commun. 1996 Aug 14;225(2):377-83. doi: 10.1006/bbrc.1996.1183.
We here show the application of mRNA differential display to investigate changes in gene expression in rat liver cirrhosis and address problems inherent in the technique when applied to this complex disease model. A number of differentially expressed mRNA species could be identified and two were analyzed in more detail here. One was found to derive from a new gene while the other corresponded to fetuin, a 41 kDa N-glycoprotein that specifically inhibits tyrosine kinase activity of the insulin receptor when phosphorylated. Fetuin expression was reduced by 45% in liver cirrhosis induced by bile duct ligation, but not in cirrhosis induced by carbon tetrachloride/Phenobarbital, as compared to controls. Our results raise the possibility that fetuin plays a regulatory role in the proliferation of parenchymal liver cells.
我们在此展示了mRNA差异显示技术在研究大鼠肝硬化基因表达变化中的应用，并探讨了该技术应用于这种复杂疾病模型时所固有的问题。可以鉴定出许多差异表达的mRNA种类，这里对其中两种进行了更详细的分析。一种被发现来自一个新基因，另一种对应于胎球蛋白，一种41 kDa的N-糖蛋白，磷酸化时可特异性抑制胰岛素受体的酪氨酸激酶活性。与对照组相比，在胆管结扎诱导的肝硬化中胎球蛋白表达降低了45%，但在四氯化碳/苯巴比妥诱导的肝硬化中未降低。我们的结果提出了胎球蛋白在肝实质细胞增殖中起调节作用的可能性。
