草地贪夜蛾细胞中的类信号转导和转录激活因子DNA结合活性
STAT-like DNA-binding activity in Spodoptera frugiperda cells.
作者信息
Sliva D, Haldosén L A
机构信息
Department of Medical Nutrition, Karolinska Institute, Huddinge, Sweden.
出版信息
Biochem Biophys Res Commun. 1996 Aug 14;225(2):562-9. doi: 10.1006/bbrc.1996.1212.
Nuclear extracts from Spodoptera frugiperda (Sf9) cells were shown to contain a factor binding to DNA elements with gamma interferon activated site-like sequences. The DNA-binding activity was shown to be dependent on tyrosine phosphorylation. Hydrodynamic characterization of this entity revealed a Stokes radius of 8.4 nm and a sedimentation coefficient of 5.9 S. The molecular weight was calculated to 209,000. Estimated frictional (f/to) and axial (a/b) ratios indicated an elongated structure of this DNA-binding entity. This DNA-binding factor could represent a dimer of a Sf9 homolog to the mammalian signal transducers and activators of transcription.
已证明，草地贪夜蛾（Sf9）细胞的核提取物含有一种与具有γ干扰素激活位点样序列的DNA元件结合的因子。该DNA结合活性显示依赖于酪氨酸磷酸化。对该实体的流体动力学表征显示，其斯托克斯半径为8.4纳米，沉降系数为5.9 S。计算出的分子量为209,000。估计的摩擦（f/to）和轴比（a/b）表明该DNA结合实体具有细长结构。这种DNA结合因子可能代表哺乳动物信号转导和转录激活因子的Sf9同源物的二聚体。
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