Mulligan P K, Campos A R, Jacobs J R
McMaster University, Department of Biology, Hamilton, Ontario, Canada.
Dev Genet. 1996;18(4):316-24. doi: 10.1002/(SICI)1520-6408(1996)18:4<316::AID-DVG5>3.0.CO;2-C.
During oogenesis in Drosophila, germ cells appear in sequential clusters of 16 interconnected cells. The events surrounding the differentiation of these cells are not fully understood. Here we present genetic and morphological analysis of mutations in the gene stand still (stil). Through complementation analyses we have refined the location of this gene to cyological region 49B-C. Our analyses of ovaries from ethylmethane sulfonate (EMS)-induced mutant alleles of this gene suggest that mutations in the stil gene produce a wide range of phenotypic abnormalities, from the absence of germ cells in the most severe alleles, to egg chambers with cytoskeletal defects in the less severe alleles. Our results suggest a role for this gene in specifying or maintaining a cytoskeletal component, with consequences during oogenesis and possibly during germ line sex determination.
在果蝇卵子发生过程中，生殖细胞以16个相互连接的细胞组成的连续簇状出现。围绕这些细胞分化的事件尚未完全了解。在此，我们展示了静止基因（stil）突变的遗传和形态学分析。通过互补分析，我们已将该基因的位置精确定位于细胞学区域49B - C。我们对该基因的乙基甲磺酸酯（EMS）诱导突变等位基因的卵巢分析表明，stil基因突变会产生广泛的表型异常，从最严重等位基因中生殖细胞的缺失，到较不严重等位基因中具有细胞骨架缺陷的卵室。我们的结果表明该基因在指定或维持细胞骨架成分方面起作用，在卵子发生过程中以及可能在生殖系性别决定过程中产生影响。
