Sakharova N Iu, Malashenko A M, Mezhevikina L M, Lepikhov K A, Fialkovskaia L A
Ontogenez. 1996 May-Jun;27(3):232-5.
We studied early embryonic mortality of mice from mutant stocks Tabby (Ta, X-chromosome) and RaSd (RaSd/++, chromosome 2) maintained in the heterozygous state in F1 CBA x C57B1/6 hybrid. Tabby and RaSd mice were reciprocally crossed with F1 mice and examined for the morphological status of embryos washed from the oviduct on the third day of pregnancy, when the stage of eight blastomeres is normally attained. Mortality was evaluated from the number of embryos which did not reach the expected stage by this time. The results have shown that 2-4 cell embryos, which have received gene Ta with the X-chromosome of the female parent, differed from embryos with F1 genotype at the same stage of development by their increased mortality rate, whereas among embryos obtained from RaSd, the mortality was mainly observed before cleavage. Death of embryos receiving the mutant gene from hemizygous Ta males or heterozygous RaSd/++ males was not significantly different from the mortality of embryos without these mutations.
我们研究了在F1 CBA×C57B1/6杂种中以杂合状态维持的突变品系虎斑（Ta，X染色体）和RaSd（RaSd/++，2号染色体）小鼠的早期胚胎死亡率。将虎斑和RaSd小鼠与F1小鼠进行正反交，并在妊娠第三天检查从输卵管冲洗出的胚胎的形态状态，此时正常达到八细胞期。根据此时未达到预期阶段的胚胎数量评估死亡率。结果表明，从母本接受带有基因Ta的X染色体的2-4细胞胚胎，在相同发育阶段与具有F1基因型的胚胎相比，死亡率增加，而在从RaSd获得的胚胎中，死亡率主要在卵裂前观察到。从半合子Ta雄性或杂合子RaSd/++雄性获得突变基因的胚胎死亡与没有这些突变的胚胎死亡率没有显著差异。
