水仙色素体伴侣蛋白60和热休克蛋白70的纯化与特性分析
Purification and characterization of chaperonin 60 and heat-shock protein 70 from chromoplasts of Narcissus pseudonarcissus.
作者信息
Bonk M, Tadros M, Vandekerckhove J, Al-Babili S, Beyer P
机构信息
Institut für Biologie II der Universität, Zellbiologie, Belgium.
出版信息
Plant Physiol. 1996 Jul;111(3):931-9. doi: 10.1104/pp.111.3.931.
Abstract
In chromoplast differentiation during flower formation in Narcissus pseudonarcissus, the molecular chaperones chaperonin 60 (Cpn60; alpha and beta) and heat-shock protein 70 (Hsp70) greatly increase in abundance. Both were purified and shown to be present in a functional form in chromoplasts, indicating their requirement in the extensive structural rearrangements during the chloroplast-to-chromoplast transition. The purified proteins, sequenced N terminally and from internal peptides, showed strong homology to plastid Cpn60 and Hsp 70 representatives from other plant species. During chromoplast differentiation, the carotenoid biosynthetic pathway is strongly induced. The corresponding enzymes are all nuclear encoded and form a large, soluble, hetero-oligomeric protein complex after import but prior to their membrane attachment. By immunoprecipitations we have shown that the plastid Hsp70 is a structural constituent of a soluble entity also containing phytoene desaturase.
摘要
在水仙成花过程中的质体色素分化过程中，分子伴侣伴侣蛋白60（Cpn60；α和β）和热休克蛋白70（Hsp70）的丰度大幅增加。二者均被纯化，并显示以功能形式存在于质体色素中，这表明它们在叶绿体到质体色素转变过程中的广泛结构重排中是必需的。纯化后的蛋白质经N端测序和内部肽段测序，显示与其他植物物种的质体Cpn60和Hsp 70代表具有很强的同源性。在质体色素分化过程中，类胡萝卜素生物合成途径被强烈诱导。相应的酶均由核编码，在导入后但在它们附着于膜之前形成一个大的、可溶的、异源寡聚蛋白复合体。通过免疫沉淀，我们已表明质体Hsp70是一个也含有八氢番茄红素去饱和酶的可溶实体的结构成分。
相似文献
1
Purification and characterization of chaperonin 60 and heat-shock protein 70 from chromoplasts of Narcissus pseudonarcissus.水仙色素体伴侣蛋白60和热休克蛋白70的纯化与特性分析
Plant Physiol. 1996 Jul;111(3):931-9. doi: 10.1104/pp.111.3.931.
2
A novel, soluble form of phytoene desaturase from Narcissus pseudonarcissus chromoplasts is Hsp70-complexed and competent for flavinylation, membrane association and enzymatic activation.来自水仙色素体的一种新型可溶性八氢番茄红素去饱和酶与热休克蛋白70（Hsp70）结合，具备黄素化、膜结合及酶激活能力。
Plant J. 1996 May;9(5):601-12. doi: 10.1046/j.1365-313x.1996.9050601.x.
3
Comparison of the expression of a plastidic chaperonin 60 in different plant tissues and under photosynthetic and non-photosynthetic conditions.质体伴侣蛋白60在不同植物组织以及光合和非光合条件下的表达比较。
Planta. 1996;200(3):326-36. doi: 10.1007/BF00200300.
4
Carotene desaturation is linked to a respiratory redox pathway in Narcissus pseudonarcissus chromoplast membranes. Involvement of a 23-kDa oxygen-evolving-complex-like protein.胡萝卜素去饱和作用与水仙色素体膜中的呼吸氧化还原途径有关。一种23 kDa类放氧复合体蛋白的参与。
Eur J Biochem. 1995 Nov 1;233(3):864-72. doi: 10.1111/j.1432-1033.1995.864_3.x.
5
A 77-kilodalton protein of Cryptococcus neoformans, a member of the heat shock protein 70 family, is a major antigen detected in the sera of mice with pulmonary cryptococcosis.新型隐球菌的一种77千道尔顿蛋白，属于热休克蛋白70家族成员，是在患有肺隐球菌病的小鼠血清中检测到的主要抗原。
Infect Immun. 1997 May;65(5):1653-8. doi: 10.1128/iai.65.5.1653-1658.1997.
6
Molecular chaperones are present in the thylakoid lumen of pea chloroplasts.分子伴侣存在于豌豆叶绿体的类囊体腔中。
FEBS Lett. 1996 Feb 5;379(3):302-4. doi: 10.1016/0014-5793(95)01534-5.
7
The Hsp70 and Hsp60 chaperone machines.热休克蛋白70（Hsp70）和热休克蛋白60（Hsp60）伴侣机制。
Cell. 1998 Feb 6;92(3):351-66. doi: 10.1016/s0092-8674(00)80928-9.
8
Interaction of plant mitochondrial and chloroplast signal peptides with the Hsp70 molecular chaperone.植物线粒体和叶绿体信号肽与热休克蛋白70分子伴侣的相互作用。
Trends Plant Sci. 2002 Jan;7(1):14-21. doi: 10.1016/s1360-1385(01)02180-x.
9
Chloroplast import of four carotenoid biosynthetic enzymes in vitro reveals differential fates prior to membrane binding and oligomeric assembly.体外对四种类胡萝卜素生物合成酶的叶绿体导入揭示了在膜结合和寡聚体组装之前的不同命运。
Eur J Biochem. 1997 Aug 1;247(3):942-50. doi: 10.1111/j.1432-1033.1997.00942.x.
10
Phytoene synthase from Narcissus pseudonarcissus: functional expression, galactolipid requirement, topological distribution in chromoplasts and induction during flowering.水仙的八氢番茄红素合酶：功能表达、半乳糖脂需求、在质体中的拓扑分布及开花期诱导
Plant J. 1996 Nov;10(5):781-92. doi: 10.1046/j.1365-313x.1996.10050781.x.
引用本文的文献
1
"Omics" insights into plastid behavior toward improved carotenoid accumulation.“组学”对质体行为促进类胡萝卜素积累的见解。
Front Plant Sci. 2022 Oct 6;13:1001756. doi: 10.3389/fpls.2022.1001756. eCollection 2022.
2
OsCpn60β1 is Essential for Chloroplast Development in Rice ( L.).OsCpn60β1 在水稻叶绿体发育中是必需的。
Int J Mol Sci. 2020 Jun 4;21(11):4023. doi: 10.3390/ijms21114023.
3
Manipulation of Plastidial Protein Quality Control Components as a New Strategy to Improve Carotenoid Contents in Tomato Fruit.操纵质体蛋白质量控制组件作为提高番茄果实类胡萝卜素含量的新策略。
Front Plant Sci. 2019 Sep 5;10:1071. doi: 10.3389/fpls.2019.01071. eCollection 2019.
4
Molecular Link between Leaf Coloration and Gene Expression of Flavonoid and Carotenoid Biosynthesis in Cultivar 'Huangjinya'.‘黄金芽’品种叶片着色与类黄酮和类胡萝卜素生物合成基因表达之间的分子联系
Front Plant Sci. 2017 May 24;8:803. doi: 10.3389/fpls.2017.00803. eCollection 2017.
5
Structural insight into the cooperation of chloroplast chaperonin subunits.叶绿体伴侣蛋白亚基合作的结构洞察
BMC Biol. 2016 Apr 12;14:29. doi: 10.1186/s12915-016-0251-8.
6
Carotenoid metabolism and regulation in horticultural crops.园艺作物中的类胡萝卜素代谢与调控
Hortic Res. 2015 Aug 26;2:15036. doi: 10.1038/hortres.2015.36. eCollection 2015.
7
Arabidopsis J-protein J20 delivers the first enzyme of the plastidial isoprenoid pathway to protein quality control.拟南芥 J 蛋白 J20 将质体异戊二烯途径的第一个酶递送到蛋白质质量控制。
Plant Cell. 2013 Oct;25(10):4183-94. doi: 10.1105/tpc.113.113001. Epub 2013 Oct 8.
8
Evolution of chloroplast J proteins.叶绿体 J 蛋白的进化。
PLoS One. 2013 Jul 23;8(7):e70384. doi: 10.1371/journal.pone.0070384. Print 2013.
9
OsCpn60α1, encoding the plastid chaperonin 60α subunit, is essential for folding of rbcL.OsCpn60α1，编码质体分子伴侣 60α 亚基，对于 rbcL 的折叠是必需的。
Mol Cells. 2013 May;35(5):402-9. doi: 10.1007/s10059-013-2337-2. Epub 2013 Apr 24.
10
On the structure and function of the phytoene desaturase CRTI from Pantoea ananatis, a membrane-peripheral and FAD-dependent oxidase/isomerase.关于 Pantoea ananatis 中质体番茄红素脱饱和酶 CRTI 的结构与功能，一种膜周质和 FAD 依赖的氧化酶/异构酶。
PLoS One. 2012;7(6):e39550. doi: 10.1371/journal.pone.0039550. Epub 2012 Jun 22.
本文引用的文献
1
Isolation and characterization of a cDNA clone encoding the major hsp70 of the pea chloroplastic stroma.编码豌豆叶绿体基质主要热休克蛋白70（hsp70）的cDNA克隆的分离与鉴定。
Plant Physiol. 1992 Oct;100(2):1048-54. doi: 10.1104/pp.100.2.1048.
2
Alterations in the phenotype of plant cells studied by NH(2)-terminal amino acid-sequence analysis of proteins electroblotted from two-dimensional gel-separated total extracts.用电转移技术从二维凝胶分离的总提取物中分离蛋白质，通过对其 N 端氨基酸序列的分析，研究植物细胞表型的变化。
Proc Natl Acad Sci U S A. 1987 Jul;84(14):4806-10. doi: 10.1073/pnas.84.14.4806.
3
THE ANATOMY OF THE TOBACCO MOSAIC VIRUS.烟草花叶病毒的剖析
Virology. 1964 Mar;22:342-59. doi: 10.1016/0042-6822(64)90025-x.
4
Newly Imported Rieske Iron-Sulfur Protein Associates with Both Cpn60 and Hsp70 in the Chloroplast Stroma.新导入的 Rieske 铁硫蛋白与叶绿体基质中的 Cpn60 和 Hsp70 均相关联。
Plant Cell. 1993 Dec;5(12):1865-1876. doi: 10.1105/tpc.5.12.1865.
5
Involvement of a chloroplast HSP70 heat shock protein in the integration of a protein (light-harvesting complex protein precursor) into the thylakoid membrane.一种叶绿体HSP70热休克蛋白参与将一种蛋白质（捕光复合体蛋白前体）整合到类囊体膜中。
Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5616-9. doi: 10.1073/pnas.89.12.5616.
6
Reconstitution of active dimeric ribulose bisphosphate carboxylase from an unfoleded state depends on two chaperonin proteins and Mg-ATP.从无折叠状态重构活性二聚体核酮糖二磷酸羧化酶依赖于两种伴侣蛋白和Mg-ATP。
Nature. 1989;342(6252):884-9. doi: 10.1038/342884a0.
7
Isolation of the Rieske iron-sulfur protein from the cytochrome b6/f complex of spinach chloroplasts.从菠菜叶绿体的细胞色素b6/f复合体中分离 Rieske 铁硫蛋白。
FEBS Lett. 1981 Nov 2;134(1):1-5. doi: 10.1016/0014-5793(81)80537-6.
8
A novel, soluble form of phytoene desaturase from Narcissus pseudonarcissus chromoplasts is Hsp70-complexed and competent for flavinylation, membrane association and enzymatic activation.来自水仙色素体的一种新型可溶性八氢番茄红素去饱和酶与热休克蛋白70（Hsp70）结合，具备黄素化、膜结合及酶激活能力。
Plant J. 1996 May;9(5):601-12. doi: 10.1046/j.1365-313x.1996.9050601.x.
9
Stromal factor plays an essential role in protein integration into thylakoids that cannot be replaced by unfolding or by heat shock protein Hsp70.基质因子在蛋白质整合到类囊体中起着至关重要的作用，这种作用无法通过蛋白质解折叠或热休克蛋白Hsp70来替代。
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8552-6. doi: 10.1073/pnas.90.18.8552.
10
Interaction of homologues of Hsp70 and Cpn60 with ferredoxin-NADP+ reductase upon its import into chloroplasts.Hsp70和Cpn60的同源物在铁氧还蛋白-NADP⁺还原酶导入叶绿体过程中与其发生的相互作用。
FEBS Lett. 1993 Apr 12;320(3):198-202. doi: 10.1016/0014-5793(93)80585-i.
Zotero 插件