thor Straten P, Becker J C, Seremet T, Bröcker E B, Zeuthen J
Department of Tumor Cell Biology, Division of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark.
J Clin Invest. 1996 Jul 15;98(2):279-84. doi: 10.1172/JCI118790.
The T cell receptor (TCR) BV variable (V) gene repertoire of tumor infiltrating lymphocytes (TIL) found in progressive and regressive regions of the same primary human melanomas were characterized by reverse transcription coupled polymerase chain reaction (RT-PCR). After surgery, the tumors were divided into different parts which were judged as regressive or progressive regions by visual inspection. Subsequently this diagnosis was confirmed by histology. From a total of four primary melanomas analyzed, 2 were drawn to be HLA-A2+. Only relatively few BV-gene families were expressed at significant levels in each of the samples. Comparison of the BV-expression in regressive versus progressive regions of the same tumor revealed major differences in all cases examined. Direct sequencing of RT-PCR products indicated that highly expressed BV-gene families were of clonal origin in both the regressive and progressive regions. Together, these data strongly suggest the occurrence of clonal T cell responses in both regressive and progressive areas of the same primary tumor. The differences in expression of certain BV-genes may correlate with the functional activity of certain populations of tumor-infiltrating T cells.
采用逆转录聚合酶链反应（RT-PCR）对同一原发性人类黑色素瘤进展期和消退期肿瘤浸润淋巴细胞（TIL）的T细胞受体（TCR）BV可变（V）基因库进行了表征。手术后，将肿瘤分成不同部分，通过肉眼检查判断为消退或进展区域。随后，这一诊断通过组织学得到证实。在总共分析的4例原发性黑色素瘤中，有2例被判定为HLA - A2 +。在每个样本中，只有相对较少的BV基因家族有显著表达。同一肿瘤消退区与进展区BV表达的比较显示，在所有检查的病例中都存在主要差异。RT-PCR产物的直接测序表明，在消退区和进展区中，高表达的BV基因家族均来源于克隆。这些数据共同有力地表明，在同一原发性肿瘤的消退区和进展区均发生了克隆性T细胞反应。某些BV基因表达的差异可能与某些肿瘤浸润T细胞群体的功能活性相关。
