通过单个氨基酸取代，将大肠杆菌中的一种次要硝基还原酶NfsB转化为一种生化特性与费氏弧菌中的主要黄素还原酶FRase I相似的黄素还原酶。
Conversion of NfsB, a minor Escherichia coli nitroreductase, to a flavin reductase similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri, by a single amino acid substitution.
作者信息
Zenno S, Koike H, Tanokura M, Saigo K
机构信息
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
出版信息
J Bacteriol. 1996 Aug;178(15):4731-3. doi: 10.1128/jb.178.15.4731-4733.1996.
Abstract
NfsB is an oxygen-insensitive nitroreductase of Escherichia coli with significant amino acid sequence homology to the major flavin reductase (FRase I) of Vibrio fischeri. Here, we show that NfsB is convertible to an FRase I-like flavin reductase three times as active as the authentic FRase I by a single amino acid substitution in the least-conserved region.
摘要
NfsB是大肠杆菌的一种对氧不敏感的硝基还原酶，与费氏弧菌的主要黄素还原酶（FRase I）具有显著的氨基酸序列同源性。在此，我们表明，通过在保守性最低的区域进行单个氨基酸替换，NfsB可转化为一种活性是天然FRase I三倍的类FRase I黄素还原酶。
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Conversion of NfsB, a minor Escherichia coli nitroreductase, to a flavin reductase similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri, by a single amino acid substitution.通过单个氨基酸取代，将大肠杆菌中的一种次要硝基还原酶NfsB转化为一种生化特性与费氏弧菌中的主要黄素还原酶FRase I相似的黄素还原酶。
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本文引用的文献
1
Identification of the gene encoding the major NAD(P)H-flavin oxidoreductase of the bioluminescent bacterium Vibrio fischeri ATCC 7744.费氏弧菌ATCC 7744生物发光细菌主要NAD(P)H-黄素氧化还原酶编码基因的鉴定。
J Bacteriol. 1994 Jun;176(12):3536-43. doi: 10.1128/jb.176.12.3536-3543.1994.
2
3
Physical characterisation of the Escherichia coli B gene encoding nitroreductase and its over-expression in Escherichia coli K12.编码硝基还原酶的大肠杆菌B基因的物理特性及其在大肠杆菌K12中的过表达。
FEMS Microbiol Lett. 1994 Dec 1;124(2):195-202. doi: 10.1111/j.1574-6968.1994.tb07284.x.
4
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.一对用于选择双酶切限制片段任一条DNA链的新型M13载体。
Gene. 1982 Oct;19(3):269-76. doi: 10.1016/0378-1119(82)90016-6.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Production of single-stranded plasmid DNA.单链质粒DNA的制备。
Methods Enzymol. 1987;153:3-11. doi: 10.1016/0076-6879(87)53044-0.
7
8
Nucleotide sequence of Salmonella typhimurium nitroreductase gene.鼠伤寒沙门氏菌硝基还原酶基因的核苷酸序列。
Nucleic Acids Res. 1990 Feb 25;18(4):1059. doi: 10.1093/nar/18.4.1059.
9
Characterization of the flavin reductase gene (fre) of Escherichia coli and construction of a plasmid for overproduction of the enzyme.大肠杆菌黄素还原酶基因（fre）的特性鉴定及用于该酶过量表达的质粒构建。
J Bacteriol. 1991 Jun;173(12):3673-9. doi: 10.1128/jb.173.12.3673-3679.1991.
10
Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae.阴沟肠杆菌硝基还原酶基因的克隆、核苷酸序列及表达
J Biol Chem. 1991 Mar 5;266(7):4126-30.
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