Ndele J K, Yoshioka K, Fisher J W
Department of Clinical Pharmacology, College of Health Sciences, University of Nairobi.
East Afr Med J. 1996 Feb;73(2):143-6.
The present studies were designed and carried out to determine if hydrogen peroxide (H2O2) is involved in the regulation of erythropoietin (Epo) gene expression and stimulation of Epo production in the hepatocellular (Hep 3B) cells. Hep 3B cells were incubated with varying concentrations of H2O2 for periods of 6 hours or 24 hours. In other experiments Hep 3B cells were incubated for 24 hours with or without increasing concentrations of catalase and in the presence of H2O2. Culture medium levels of Epo were determined and quantitation of Epo mRNA was also made. The results indicate that H2O2 increases the levels of Epo mRNA and Epo hormone production in Hep 3B cells, and that catalase, the specific scavenger of hydrogen peroxide, inhibits Epo production in these cells. Based on these findings, it is concluded that H2O2 takes part in the signal transduction mechanisms in Epo production. It is recommended that further studies be undertaken to find out the source of the hydrogen peroxide in the hepatocellular carcinoma cells.
本研究旨在确定过氧化氢（H2O2）是否参与肝细胞（Hep 3B）中促红细胞生成素（Epo）基因表达的调控以及Epo生成的刺激过程。将Hep 3B细胞与不同浓度的H2O2孵育6小时或24小时。在其他实验中，将Hep 3B细胞在有或没有增加浓度的过氧化氢酶且存在H2O2的情况下孵育24小时。测定培养基中Epo的水平，并对Epo mRNA进行定量分析。结果表明，H2O2可增加Hep 3B细胞中Epo mRNA的水平和Epo激素的生成，而过氧化氢的特异性清除剂过氧化氢酶可抑制这些细胞中Epo的生成。基于这些发现，得出结论：H2O2参与了Epo生成的信号转导机制。建议进一步开展研究以找出肝癌细胞中过氧化氢的来源。
