Granger C L, Callos J D, Medford J I
Department of Biology, Pennsylvania State University, University Park 16802, USA.
Plant Mol Biol. 1996 May;31(2):373-8. doi: 10.1007/BF00021797.
The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolated and characterized the corresponding clone, Merihb1, from Arabidopsis. Analysis shows that the predicted MERIHB1 protein exhibits strong homology to KN1 and RS1 from maize, SBH1 from soybean, and KNAT1 and KNAT2 from Arabidopsis. Merihb1 is highly expressed in mRNA from cauliflower meristems and also accumulates in stem and flower mRNA. Based on the similarity of the Merihb1 and Kn1 sequences, expression patterns, and in situ hybridizations, we suggest that Merihb1 represents an Arabidopsis homologue of the maize Kn1 gene.
茎尖分生组织（SAM）负责形成植物地上部分的大部分结构。我们试图通过用来自玉米Knotted-1（Kn1）基因的同源异型框片段筛选甘蓝型油菜（花椰菜）分生组织cDNA文库，来分离在拟南芥茎尖分生组织中表达的调控基因。我们从拟南芥中分离并鉴定了相应的克隆Merihb1。分析表明，预测的MERIHB1蛋白与玉米的KN1和RS1、大豆的SBH1以及拟南芥的KNAT1和KNAT2具有高度同源性。Merihb1在花椰菜分生组织的mRNA中高度表达，并且在茎和花的mRNA中也有积累。基于Merihb1和Kn1序列的相似性、表达模式以及原位杂交结果，我们认为Merihb1代表了玉米Kn1基因在拟南芥中的同源基因。
