Masclans J R, Barberà J A, MacNee W, Pavia J, Piera C, Lomeña F, Chung K F, Roca J, Rodriguez-Roisin R
Department de Medicina, Hospital Clinic, Universitat de Barcelona, Spain.
Am J Respir Crit Care Med. 1996 Aug;154(2 Pt 1):529-32. doi: 10.1164/ajrccm.154.2.8756833.
To investigate whether salbutamol inhibits platelet-activating factor (PAF)-induced neutrophil sequestration in the lungs, we studied eight nonatopic, nonsmoking, healthy subjects (six men; aged 27.0 +/- 1.5 (SE) yr) with PAF-induced bronchial response. Prior to PAF challenge (24 micrograms), they inhaled either salbutamol (300 micrograms) or placebo in a randomized, double-blind, crossover manner two weeks apart. Respiratory system resistance (Rrs), arterial blood gases, and neutrophil counts were measured 4, 8, 12 and 30 min after PAF. Neutrophil kinetics in the lungs were assessed by tracking autologous 99mTc-erythrocytes and 111in-neutrophils. Compared with salbutamol, arterial blood neutrophil counts fell (p < 0.04) maximally at 4 min after PAF, followed by a mild rebound neutrophilia, whereas Rrs increased (p < 0.01) and Pao2 decreased (p < 0.05) at 4 min only. The intrapulmonary activity of 111in-neutrophils after pretreatment with placebo was higher compared with salbutamol (1.98 +/- 0.15 versus 1.33 +/- 0.23 cps/mCi/pixel) (p < 0.01) although both their initial sequestration (first-pass) and subsequent washout were not significantly different. Inhaled salbutamol blocks pulmonary neutrophil sequestration and lung function abnormalities following PAF challenge in humans.
为研究沙丁胺醇是否抑制血小板活化因子（PAF）诱导的中性粒细胞在肺内的扣押，我们研究了8名非特应性、不吸烟的健康受试者（6名男性；年龄27.0±1.5（标准误）岁），这些受试者有PAF诱导的支气管反应。在PAF激发（24微克）前，他们以随机、双盲、交叉方式每隔两周吸入沙丁胺醇（300微克）或安慰剂。在PAF激发后4、8、12和30分钟测量呼吸系统阻力（Rrs）、动脉血气和中性粒细胞计数。通过追踪自体99mTc-红细胞和111In-中性粒细胞评估肺内中性粒细胞动力学。与沙丁胺醇相比，PAF激发后4分钟动脉血中性粒细胞计数最大程度下降（p<0.04），随后有轻度的中性粒细胞增多反弹，而仅在4分钟时Rrs增加（p<0.01）且Pao2降低（p<0.05）。与沙丁胺醇相比，安慰剂预处理后111In-中性粒细胞的肺内活性更高（1.98±0.15对1.33±0.23 cps/mCi/像素）（p<0.01），尽管它们最初的扣押（首过）和随后的清除没有显著差异。吸入沙丁胺醇可阻断人类PAF激发后肺内中性粒细胞的扣押和肺功能异常。
