Mandal S S, Kumar N V, Varshney U, Bhattacharya S
Department of Organic Chemistry, Indian Institute of Science, Bangalore, India.
J Inorg Biochem. 1996 Sep;63(4):265-72. doi: 10.1016/0162-0134(95)00228-6.
A new water-soluble, salen [salen = bis(salicylidene) ethylenediamine]-based ligand, 3 was developed. Two of the metal complexes of this ligand, i.e., 3a, [Mn(III)] and 3b, [Ni(II)], in the presence of cooxidant magnesium monoperoxyphthalate (MMPP) cleaved plasmid DNA pTZ19R efficiently and rapidly at a concentration approximately 1 microM. In contrast, under comparable conditions, other metal complexes 3c, [Cu(II)] or 3d, [Cr(III)] could not induce any significant DNA nicking. The findings with Ni(II) complex suggest that the DNA cleavage processes can be modulated by the disposition of charges around the ligand.
一种新型的基于水杨醛缩乙二胺（salen = 双（水杨醛）乙二胺）的水溶性配体3被研发出来。该配体的两种金属配合物，即3a，[Mn(III)] 和3b，[Ni(II)]，在共氧化剂单过氧邻苯二甲酸镁（MMPP）存在的情况下，能在大约1微摩尔的浓度下高效且快速地切割质粒DNA pTZ19R。相比之下，在类似条件下，其他金属配合物3c，[Cu(II)] 或3d，[Cr(III)] 不会引起任何明显的DNA切口。镍（II）配合物的研究结果表明，DNA切割过程可以通过配体周围电荷的分布来调节。
