Agrawal B, Reddish M A, Longenecker B M
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.
J Immunol. 1996 Sep 1;157(5):2089-95.
We have recently provided evidence that there is a natural immunization against human cancer-associated MUC-1 mucin epitopes during pregnancy by studying MUC-1 Ag-specific T cell lines established from multiparous women. Using this experimental model system, we now report that MUC-1 peptide-specific MHC class I-restricted CTLs can be generated in vitro using T cells from multiparous women stimulated with synthetic MUC-1 peptide-loaded, autologous APCs. The complexity of cytokines produced in response to the MUC-1 peptide by anti-MUC-1 T-cells was examined. IFN-gamma was generated by MUC-1-specific T cell lines in long term cultures, whereas in short term cultures, both IFN-gamma and IL-4 were produced. The presence of MUC-1-reactive T cells in multiparous women is consistent with their potential role in immune surveillance and provides a rationale for the use of certain synthetic MUC-1 peptides for active specific immunotherapy of human carcinomas.
最近，我们通过研究从经产妇建立的MUC-1抗原特异性T细胞系，提供了证据表明在妊娠期间存在针对人类癌症相关MUC-1粘蛋白表位的天然免疫。利用这个实验模型系统，我们现在报告，使用来自经产妇的T细胞，用负载合成MUC-1肽的自体抗原呈递细胞（APC）刺激，可以在体外产生MUC-1肽特异性MHC I类限制性细胞毒性T淋巴细胞（CTL）。研究了抗MUC-1 T细胞对MUC-1肽产生的细胞因子的复杂性。在长期培养中，MUC-1特异性T细胞系产生γ干扰素（IFN-γ），而在短期培养中，同时产生IFN-γ和白细胞介素-4（IL-4）。经产妇中存在MUC-1反应性T细胞与其在免疫监视中的潜在作用一致，并为使用某些合成MUC-1肽进行人类癌症的主动特异性免疫治疗提供了理论依据。
