Kirpekar F, Gulløv K
Department of Molecular Biology, Odense University, Denmark.
Mol Gen Genet. 1996 Jul 26;251(6):716-9. doi: 10.1007/BF02174121.
The replication behaviour of a number of ARS1-based plasmids was investigated on propagation in Saccharomyces cerevisiae grown with either glucose or galactose as carbon source. Growth on galactose results in reduced plasmid stability, as well as in reduced replication efficiency, when the entire 1.5-kb TRP1-ARS1 fragment is present on a plasmid. The galactose sensitivity is mediated by a 0.13-kb fragment harbouring part of the GAL3 promoter. This fragment exerts its effect when situated either 5' or 3' to the ARS core consensus at distances up to 0.9 kb. The endogenous 2 microns plasmid remained unaffected by the choice of carbon source.
研究了一些基于ARS1的质粒在以葡萄糖或半乳糖作为碳源生长的酿酒酵母中传代时的复制行为。当整个1.5 kb的TRP1-ARS1片段存在于质粒上时，在半乳糖上生长会导致质粒稳定性降低以及复制效率降低。半乳糖敏感性由一个包含GAL3启动子部分的0.13 kb片段介导。该片段位于ARS核心共有序列的5'或3'端，距离可达0.9 kb时发挥其作用。内源性2μm质粒不受碳源选择的影响。
