Davies R L, Donachie W
Division of Infection and Immunity, IBLS, University of Glasgow, UK.
Microbiology (Reading). 1996 Jul;142 ( Pt 7):1895-907. doi: 10.1099/13500872-142-7-1895.
Intra-specific diversity within Pasteurella haemolytica was assessed by analysing variation in the capsular polysaccharide (serotypes), lipopolysaccharide (LPS) and outer-membrane proteins (OMPs) of 184 isolates recovered from cattle and sheep. Four 12 serotypes comprised 83% of the total number of isolates, including A1 and A2 as the most frequently recovered serotypes from cattle and sheep, respectively. Nine distinct LPS profiles were identified. Four different core-oligosaccharide patterns were present, each of which occurred alone as rough LPS and also in association with single O-antigen profile as smooth LPS; the ninth LPS type was also smooth but had a different O-antigen profile. The capsular serotypes could be divided into four groups based on the dominant LPS profile within each serotype: (1) A1, A6, A9, A12 and A5; (2) A2, A8, A14 and A16; (3) A7 and A13; and (4) A11. Smooth LPS of type 1A, which was found only in the first group, was associated primarily with bovine disease isolates, whereas rough LPS of types 1B and 3B were associated primarily with group 2 serotypes and ovine disease isolates. Similarly, the variation of OMP profiles generated three groups: (1) A1, A6 A9, A12, A5 and A8; (2) A2, A14 and A16; and (3) A7, A11 and A13. Isolates belonging to groups 2 and 3 exhibited greater diversity in their OMP profiles than those belonging to group 1. Although the majority of group 3 isolates possessed profiles unique to that group, a smaller number of A7 isolates possessed profiles with similarities to those of serotypes A1 or A2. OMP profiles clearly differentiated bovine from ovine isolates of the same serotypes. The association both of specific LPS and OMP profiles with bovine or ovine disease isolates suggested a correlation between specific cell-surface structures and host specificity. The combined analysis of capsular serotypes, LPS types and OMP profiles identified seven major groups within P. haemolytica which were responsible for 59% of the disease cases, suggesting a clonal structure for this species. Overall, comparison of the capsular serotypes, LPS types and OMP profiles proved extremely useful for assessing diversity within P. haemolytica.
通过分析从牛和羊身上分离出的184株溶血巴斯德菌的荚膜多糖（血清型）、脂多糖（LPS）和外膜蛋白（OMPs）的变异情况，对溶血巴斯德菌的种内多样性进行了评估。4种血清型中的12种血清型占分离株总数的83%，其中A1和A2分别是从牛和羊身上最常分离出的血清型。鉴定出9种不同的LPS图谱。存在4种不同的核心寡糖模式，每种模式单独以粗糙LPS形式出现，也与单一O抗原图谱结合以光滑LPS形式出现；第9种LPS类型也是光滑的，但具有不同的O抗原图谱。根据每种血清型中占主导地位的LPS图谱，荚膜血清型可分为4组：（1）A1、A6、A9、A12和A5；（2）A2、A8、A14和A16；（3）A7和A13；（4）A11。仅在第一组中发现的1A型光滑LPS主要与牛病分离株相关，而1B型和3B型粗糙LPS主要与第2组血清型和羊病分离株相关。同样，OMP图谱的变异产生了3组：（1）A1、A6、A9、A12、A5和A8；（2）A2、A14和A16；（3）A7、A11和A13。属于第2组和第3组的分离株在OMP图谱上比属于第1组的分离株表现出更大的多样性。虽然第3组的大多数分离株具有该组特有的图谱，但少数A7分离株具有与A1或A2血清型相似的图谱。OMP图谱清楚地将相同血清型的牛分离株和羊分离株区分开来。特定的LPS和OMP图谱与牛或羊病分离株的关联表明特定细胞表面结构与宿主特异性之间存在相关性。对荚膜血清型、LPS类型和OMP图谱的综合分析确定了溶血巴斯德菌中的7个主要组，这些组占病例的59%，表明该物种具有克隆结构。总体而言，荚膜血清型、LPS类型和OMP图谱的比较被证明对评估溶血巴斯德菌的多样性非常有用。
