Rozinov M N, Fields B N
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
J Gen Virol. 1996 Jul;77 ( Pt 7):1425-9. doi: 10.1099/0022-1317-77-7-1425.
Interference of wild-type reovirus growth by some temperature-sensitive (ts) mutant viruses under non-permissive conditions or by other wild-type isolates has been demonstrated; however, the stage of the virus replication cycle at which interference occurs has not been defined. Examination of the time-course of the yields of T1 Lang (T1L) dsRNA in the progeny of mixed infections of T1L with T3 Dearing (T3D) or with a panel of T3D ts mutants at a non-permissive temperature revealed that interference takes place by 8-10 h post-infection and occurs prior to or at the same time as accumulation of reovirus dsRNA. Taken together with our previous results, these data indicate that interference occurs during a window between virus uncoating and synthesis of dsRNA in the reovirus replication cycle, probably at the stage of assembly of primary reovirus particles.
已经证明，在非允许条件下，一些温度敏感（ts）突变病毒或其他野生型分离株会干扰野生型呼肠孤病毒的生长；然而，干扰发生在病毒复制周期的哪个阶段尚未明确。在非允许温度下，对T1朗（T1L）与T3迪尔林（T3D）或一组T3D ts突变体进行混合感染的子代中T1L双链RNA（dsRNA）产量的时间进程进行检测，结果显示，干扰在感染后8 - 10小时发生，且在呼肠孤病毒dsRNA积累之前或同时发生。结合我们之前的结果，这些数据表明，干扰发生在呼肠孤病毒复制周期中病毒脱壳与dsRNA合成之间的一个时间段内，可能发生在初级呼肠孤病毒颗粒组装阶段。
