用光敏剂Ⅱ和氩离子激光对小鼠皮下肿瘤进行光动力治疗。

Photodynamic treatment of subcutaneous tumors in mice with photofrin II and Argon ion laser.

作者信息

You J S, Hau D M, Chen G T, Huang H F, Chen W C, Lin I H

机构信息

Institute of Radiation Biology, National Tsing Hua University, Taiwan.

出版信息

Chin Med J (Engl). 1996 Jan;109(1):83-8.

PMID:8758377

Abstract

OBJECTIVE

To investigate the combined effects of photofrin (Pf) II and laser irradiation on mice bearing subcutaneous tumors.

METHOD

Ehrlish ascites tumor cells (1 x 10(7)) were implanted subcutaneously into the breast area of ICR mice. The tumors, when grew up to 0.55 +/- 0.15cm3, was administered of laser irradiation (514.5nm, 112.5mW/cm2 for 11.12 min with a total energy 75j/cm2) after intraperitoneal injection of Pf II (5, 7.5, 10 and 15mg/kg body weight) once a day for three successive days.

RESULTS

The mortality rate (MR120) in the tumor control was 93.7% and the mean survival time (MST120) was 46.6 days. The best therapeutic effect (50% mortality rate, 53.8% tumor control rate and mean survival time 86 days) was observed in the group treated with 7.5mg of Pf II per kg of body weight and total irradiation energy of 75J/cm2 (112.5mW/cm2 for 11.12 min) with 514.5nm argon ion laser light.

CONCLUSIONS

The effect on the survival rate of tumor cells and synthetic rates of DNA, RNA and protein were obviously inhibited, as the dose of pf II and laser irradiation increased.

摘要

目的

研究血卟啉（Pf）Ⅱ与激光照射对荷皮下肿瘤小鼠的联合作用。

方法

将艾氏腹水瘤细胞（1×10⁷）皮下接种于ICR小鼠的乳腺区域。待肿瘤长至0.55±0.15cm³时，连续3天每天腹腔注射PfⅡ（5、7.5、10和15mg/kg体重），之后用514.5nm激光照射（112.5mW/cm²，照射11.12分钟，总能量75j/cm²）。

结果

肿瘤对照组的死亡率（MR120）为93.7%，平均生存时间（MST120）为46.6天。在每千克体重注射7.5mg PfⅡ且总照射能量为75J/cm²（112.5mW/cm²，照射11.12分钟）并用514.5nm氩离子激光照射的组中观察到最佳治疗效果（死亡率50%，肿瘤控制率53.8%，平均生存时间86天）。