[Anti-tumor effects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells].

OBJECTIVE

To study anti-tumor affects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells.

METHODS

The fragment of monoclonal antibody HAb18F(ab')2 which has high affinity to hepatoma cells and the fragment of monoclonal antibody antimonocytes/macrophages MAb7F(ab')2 were cross-linked with N-succinimidyl 3-(2-pyridl-dithil) propionate.

RESULTS

Heteroaggregated monoclonal antibodies HAb18F(ab')2-MAb7F(ab')2 could bind to hepatoma cells as well as monocytes-macrophages. They were purified with FPLC Sephacryl S-200. The bispecific character was identified with Indirect Immunofluorescent Staining.

CONCLUSION

In vitro cytotoxicity assay showed that HAb18F(ab')2-MAb7F(ab')2 could significantly enhance the lysis of human hepatoma cells by human monocytes-macrophages.