Wang Q, Chen Z, Wang W
Fourth Military Medical University, Xi'-an.
Zhonghua Yi Xue Za Zhi. 1996 Feb;76(2):96-9.
To study anti-tumor affects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells.
The fragment of monoclonal antibody HAb18F(ab')2 which has high affinity to hepatoma cells and the fragment of monoclonal antibody antimonocytes/macrophages MAb7F(ab')2 were cross-linked with N-succinimidyl 3-(2-pyridl-dithil) propionate.
Heteroaggregated monoclonal antibodies HAb18F(ab')2-MAb7F(ab')2 could bind to hepatoma cells as well as monocytes-macrophages. They were purified with FPLC Sephacryl S-200. The bispecific character was identified with Indirect Immunofluorescent Staining.
In vitro cytotoxicity assay showed that HAb18F(ab')2-MAb7F(ab')2 could significantly enhance the lysis of human hepatoma cells by human monocytes-macrophages.
研究靶向人单核细胞-巨噬细胞的双特异性单克隆抗体对肝癌细胞的抗肿瘤作用。
将对肝癌细胞具有高亲和力的单克隆抗体HAb18F(ab')2片段与抗单核细胞/巨噬细胞单克隆抗体MAb7F(ab')2片段用N-琥珀酰亚胺基-3-(2-吡啶二硫基)丙酸交联。
异源聚集单克隆抗体HAb18F(ab')2-MAb7F(ab')2能与肝癌细胞以及单核细胞-巨噬细胞结合。用FPLC Sephacryl S-200对其进行纯化。通过间接免疫荧光染色鉴定其双特异性特征。
体外细胞毒性试验表明，HAb18F(ab')2-MAb7F(ab')2能显著增强人单核细胞-巨噬细胞对人肝癌细胞的杀伤作用。
