[Inhibitory effects of antisense oligonucleotides delivered locally via hydrogel catheter on myointimal hyperplasia of rat carotid artery after nitrogen drying].

OBJECTIVE

To determine if antisense c-myb delivered locally via the hydrogel catheter inhibits the development of myointimal hyperplasia in rat carotid artery after nitrogen desiccation of the endothelium.

METHODS

Forty rats were randomized into four groups (ten in each). After endothelial injury, different oligonucleotides were administered locally through the hydrogel catheter. Animals were killed at 14 days following gas drying. The lumen areas, ratios of intimal to medial areas and intimal to medial thickness were measured with the aid of computer. The q test was used for statistical analysis.

RESULTS

The lumen area of antisense c-myb group (0.29 +/- 0.01 mm2) was larger than that of sense and mismatch groups (0.17 +/- 0.05 mm2, q = 4.1729; 0.18 +/- 0.05 mm2, q = 3.7489, P < 0.05), and less than that of control group (0.40 +/- 0.14, q = 3.7782, P < 0.05). The ratios of intimal to medial areas and intimal to medial thickness for antisense c-myb group (0.45 +/- 0.24, 0.57 +/- 0.24) were significantly decreased compared with sense and mismatch groups (1.26 +/- 0.25, q = 9.8913; 1.16 +/- 0.38, q = 8.4252; 2.15 +/- 0.85, q = 8.6850; 1.85 +/- 0.79, q = 6.8389, P < 0.01).

CONCLUSIONS

Myointimal hyperplasia of rat carotid artery after nitrogen drying can be markedly suppressed by intravascular local delivery of synthetic antisense c-myb via hydrogel catheter. The technique is a promising to prevent artery from obstructive stenosis.