[Study on asialoglycoprotein receptor mediated endocytosis of antiviral conjugate].

OBJECTIVE

To observe the internalization process of asialoglycoprotein receptor mediated antiviral conjugate lactosaminated human albumin arabinoside adenine monophosphate (L-HSA-Ara-AMP) in human hepatoma cell line HepG-2, and provide the specificity and practicability of receptor targeting treatment of viral hepatitis.

METHODS

Colloidal gold labelling technique combined with electronmicroscopic observation was employed. The study was carried out in three groups: experiment group (the internalization of antiviral conjugate in HepG-2 cell line), positive control group (lactosaminated human serum albumin), and negative control group and (human serum albumin).

RESULTS

L-HSA-Ara-AMP can be specially recognized and uptaken by the cells and enter the cells via coated pit. After internalization, the antiviral conjugate entered the lysosome ultimately.

CONCLUSIONS

The therapy of asialoglycoprotein receptor targeting treatment of viral hepatitis has a high specificity. The therapy is worth further study.