Cotty F, Descamps P, Ferrer A, Duong T H, Richard-Lenoble D
Service de Parasitologie et Médecine Tropicale, Hôpital Bretonneau, Tours, France.
Parasite. 1996 Jun;3(2):119-23. doi: 10.1051/parasite/1996032119.
For a better preservation and identification of Toxoplasma gondii isolates, we propose a new method of freezing of toxoplasma growth in THP-1 cell culture. A cystogenic strain isolated from foetal blood has been grown in these cells and frozen in liquid nitrogen. After thawing, toxoplasma recover the same growth rate and morphology in vitro and the same capacity to form brain cysts into mice compared to the initial strains. The freezing of the cell suspension provides a simple and appropriate method for preservation of Toxoplasma gondii within "bank" isolates.
为了更好地保存和鉴定刚地弓形虫分离株，我们提出了一种在THP-1细胞培养物中冷冻弓形虫生长的新方法。从胎儿血液中分离出的一种产囊菌株已在这些细胞中培养并在液氮中冷冻。解冻后，与初始菌株相比，弓形虫在体外恢复相同的生长速率和形态，并且在小鼠体内形成脑囊肿的能力相同。细胞悬液的冷冻为在“库”分离株中保存刚地弓形虫提供了一种简单而合适的方法。
