通过原位核酸杂交技术对单纯疱疹病毒1型角膜潜在感染的研究。

A study on HSV-1 corneal potential infection by in situ nucleic acid hybridization.

作者信息

Li S, Xie L, Dong X

机构信息

Institute of Ophthalmology, Shandong Academy of Medical Sciences, Qingdao, China.

出版信息

Yan Ke Xue Bao. 1995 Sep;11(3):117-9.

PMID:8758835

Abstract

PURPOSE

To evaluate the possibility of HSV-1 corneal latency by in situ nucleic acid hybridization in animal models.

METHODS

20 normal New Zealand White (NEW) rabbits were used, 14 of them were inoculated bilaterally with 3 x 10 PFU/ml of McKrae strain HSV-1 by intrastromal injection. 22/28 eyes developed typical herpes simplex keratitis (HSK) diseases. At 60 day postoperation (PI), 4 latent corneas were transplanted to one eye of 4 noninfected NZW rabbits and removed 2 weeks PI. Corneas at all time intervals of infection and two weeks after PKP were detected for presence of HSV-1 antigen and nucleic acid sequences by using clonal IgG HSV-1 antibody and biotinylated HSV-1 DNA probe individually.

RESULTS

The results showed that the HSV-1 DNA sequences were retained within the corneal epithelium and anterior stromal keratocytes during acute diseases, while the corneas during latent infection and postoperation, the HSV-1 DNA sequences were retained only within the stromal layer with negative HSV-1 antigen staining.

CONCLUSIONS

These results strongly suggest that the cornea may be capable of harboring latent HSV-1.

摘要

目的

通过原位核酸杂交在动物模型中评估单纯疱疹病毒1型（HSV-1）角膜潜伏的可能性。

方法

使用20只正常的新西兰白兔（NEW），其中14只通过基质内注射双侧接种3×10 PFU/ml的McKrae株HSV-1。22/28只眼发生了典型的单纯疱疹性角膜炎（HSK）疾病。术后60天（PI），将4只潜伏角膜移植到4只未感染的新西兰白兔的一只眼中，并在移植后2周取出。使用克隆IgG HSV-1抗体和生物素化的HSV-1 DNA探针分别检测感染的所有时间间隔以及穿透性角膜移植术后2周的角膜中HSV-1抗原和核酸序列的存在情况。