Nakagawa S, Watanabe M, Inoue Y
Department of Anatomy, Hokkaido University School of Medicine, Sapporo 060, Japan.
Eur J Neurosci. 1996 Jul;8(7):1401-7. doi: 10.1111/j.1460-9568.1996.tb01602.x.
The Purkinje cells in the staggerer mutant mouse have various cellular abnormalities, including reduced cell number, ectopia, smaller size and absence of dendritic spines. It is also know that some of these abnormalities exhibit regional variations in the cerebellum. In this paper we have investigated expression in the staggerer Purkinje cells of the calbindin and inositol 1,4, 5-trisphosphate receptor type 1 mRNAs by in situ hybridization. Although the transcription levels of both mRNAs were significantly reduced compared with the wild-type cells, the reduction among the Purkinje cell populations was not even, varying greatly from region to region. Purkinje cells with different transcription levels were distributed in discrete regions and arranged alternately in the mediolateral direction. Moreover, the cell bodies with higher transcription levels were larger in size and aligned in a monolayer between the granular and molecular layers, whereas those with lower levels were smaller in size, fewer in number and dispersed throughout the granular layer. These findings suggest that there is a distinct mediolateral heterogeneity in the staggerer cerebellum with respect to transcription levels of these Purkinje cell-specific molecules, which might correlate with some cytological phenotypes.
蹒跚突变小鼠的浦肯野细胞存在多种细胞异常，包括细胞数量减少、异位、体积较小以及缺乏树突棘。还已知其中一些异常在小脑中呈现区域差异。在本文中，我们通过原位杂交研究了钙结合蛋白和1,4,5-三磷酸肌醇受体1型mRNA在蹒跚突变小鼠浦肯野细胞中的表达。尽管与野生型细胞相比，这两种mRNA的转录水平均显著降低，但浦肯野细胞群体中的降低并不均匀，区域间差异很大。转录水平不同的浦肯野细胞分布在离散区域，并在内外侧方向交替排列。此外，转录水平较高的细胞体体积较大，排列在颗粒层和分子层之间的单层中，而转录水平较低的细胞体体积较小、数量较少且分散在整个颗粒层中。这些发现表明，在蹒跚突变小鼠的小脑中，就这些浦肯野细胞特异性分子的转录水平而言，存在明显的内外侧异质性，这可能与某些细胞学表型相关。
