Bora N S, Bora P S, Kaplan H J
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63110, USA.
Invest Ophthalmol Vis Sci. 1996 Aug;37(9):1870-6.
To establish a correlation between the presence of a 36 kDa protein in the blood of patients with pars planitis and to characterize and purify this protein.
Blood samples were obtained from patients with pars planitis and other types of uveitis and from various controls. Samples were treated with polyethelene glycol and protein A and were analyzed on 10% SDS-PAGE for the presence of a 36 kDa protein. Quantitative estimation of the level of this protein was determined by densitometric tracing of the stained gels. Polyclonal antibodies were raised by immunizing New Zealand White rabbits with a mixture of the gel fragment containing the 36 kDa protein (p-36) and complete Freund's adjuvant. These antibodies were used in the immunoaffinity purification of this protein.
The levels of p-36 were sixfold to eightfold higher in 81% of the patients with active pars planitis than in controls (P < 0.05). Furthermore, the levels of this protein correlated with disease activity. A partial amino terminal sequence analysis revealed that p-36 may be a novel protein. It has been purified from the patient's blood using affinity chromatography.
A 36 kDa protein (p-36) is found in elevated concentrations in the blood of many patients with active pars planitis. Its putative role in the etiopathogenesis of pars planitis is unknown.
建立中间葡萄膜炎患者血液中36 kDa蛋白的存在情况之间的相关性，并对该蛋白进行表征和纯化。
从中间葡萄膜炎患者、其他类型葡萄膜炎患者以及各类对照者中采集血样。样品用聚乙二醇和蛋白A处理，然后在10%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上分析是否存在36 kDa蛋白。通过对染色凝胶进行光密度扫描来定量测定该蛋白的水平。用含有36 kDa蛋白（p-36）的凝胶片段与完全弗氏佐剂的混合物免疫新西兰白兔，制备多克隆抗体。这些抗体用于该蛋白的免疫亲和纯化。
81%的活动性中间葡萄膜炎患者的p-36水平比对照者高6至8倍（P < 0.05）。此外，该蛋白水平与疾病活动度相关。部分氨基末端序列分析表明p-36可能是一种新蛋白。已使用亲和色谱法从患者血液中纯化出该蛋白。
在许多活动性中间葡萄膜炎患者的血液中发现36 kDa蛋白（p-36）浓度升高。其在中间葡萄膜炎发病机制中的假定作用尚不清楚。
