Lainson F A, Thomson N, Rowe H A, Langford P R, Aitchison K D, Donachie W, Kroll J S
Moredun Research Institute, Edinburgh, UK.
FEMS Microbiol Lett. 1996 Aug 15;142(1):11-7. doi: 10.1111/j.1574-6968.1996.tb08400.x.
Fifty-two ovine strains of Pasteurella haemolytica and P. trehalosi representing serotypes 1-16 were examined for the presence of [copper, zinc]superoxide dismutase DNA sequences. This was done using a combination of polymerase chain reaction with degenerate primers based on the sequence of the [Cu,Zn]superoxide dismutase gene (sodC) in related species and Southern hybridization using a fragment of sodC from P. haemolytica A2 serotype as a probe. Both detection methods identified a fragment of the sodC gene in 9/9 strains of P. haemolytica serotype 2 examined and in 5/8 strains of serotype 7. No evidence of this gene was found in any other serotype of P. haemolytica or in any P. trehalosi serotype. Comparison of DNA sequence showed near identity between sodC from the A2 and A7 serotypes of P. haemolytica and substantial similarity (70%) to sodC previously sequenced in P. multocida, Haemophilus parainfluenzae and H. influenzae. Analysis by gel electrophoresis of the superoxide dismutase activity present in cell lysates showed that one or more superoxide dismutase is present in all serotypes. However, cyanide-inhibitable activity, corresponding to [Cu,Zn]superoxide dismutase, was detected only in those strains of serotypes A2 and A7 which showed evidence of the sodC gene fragment.
对代表1 - 16血清型的52株溶血巴斯德菌和海藻糖巴斯德菌绵羊菌株进行了[铜，锌]超氧化物歧化酶DNA序列检测。采用基于相关物种中[铜，锌]超氧化物歧化酶基因（sodC）序列的简并引物进行聚合酶链反应，并以溶血巴斯德菌A2血清型的sodC片段为探针进行Southern杂交。两种检测方法均在检测的9/9株溶血巴斯德菌2型菌株和5/8株7型菌株中鉴定出sodC基因片段。在溶血巴斯德菌的任何其他血清型或海藻糖巴斯德菌的任何血清型中均未发现该基因的证据。DNA序列比较显示，溶血巴斯德菌A2和A7血清型的sodC之间几乎完全相同，与先前在多杀巴斯德菌、副流感嗜血杆菌和流感嗜血杆菌中测序的sodC具有高度相似性（70%）。对细胞裂解物中存在的超氧化物歧化酶活性进行凝胶电泳分析表明，所有血清型中均存在一种或多种超氧化物歧化酶。然而，仅在那些显示出sodC基因片段证据的A2和A7血清型菌株中检测到对应于[铜，锌]超氧化物歧化酶的氰化物抑制活性。
