欧洲醋酸杆菌和木醋杆菌的16S - 23S核糖体RNA间隔区、tRNA基因和抗终止序列。
16S-23S ribosomal RNA spacer regions of Acetobacter europaeus and A. xylinum, tRNA genes and antitermination sequences.
作者信息
Sievers M, Alonso L, Gianotti S, Boesch C, Teuber M
机构信息
Food Microbiology, ETH-Zürich, Switzerland.
出版信息
FEMS Microbiol Lett. 1996 Aug 15;142(1):43-8. doi: 10.1111/j.1574-6968.1996.tb08405.x.
The 16S-23S ribosomal RNA spacer regions of Acetobacter europaeus DSM 6160, A. xylinum NCIB 11664 and A. xylinum CL27 were amplified by PCR. Specific PCR products were obtained from each strain and their nucleotide sequences determined. The spacer region of A. europaeus comprises 768 nucleotides (nt), that of A. xylinum 778 nt and that of A. xylinum CL27 759 nt. Genes encoding tRNAIle and tRNAAla were identified. Putative antitermination sequences were found between the tRNAAla sequence and the 5'-terminus of the 23S rRNA coding sequence. The boxA element has the nucleotide sequence TGCTCTTTGATA. Based on hybridization data of digested chromosomal DNA with spacer-specific probes, the copy number of the rrn operons on the chromosome of Acetobacter strains is estimated to be four.
利用聚合酶链反应（PCR）扩增了欧洲醋杆菌DSM 6160、木醋杆菌NCIB 11664和木醋杆菌CL27的16S - 23S核糖体RNA间隔区。从每个菌株中获得了特异性PCR产物，并测定了它们的核苷酸序列。欧洲醋杆菌的间隔区由768个核苷酸（nt）组成，木醋杆菌的间隔区为778 nt，木醋杆菌CL27的间隔区为759 nt。鉴定出了编码异亮氨酸tRNA和丙氨酸tRNA的基因。在丙氨酸tRNA序列与23S rRNA编码序列的5'端之间发现了推定的抗终止序列。boxA元件的核苷酸序列为TGCTCTTTGATA。基于用间隔区特异性探针消化的染色体DNA的杂交数据，估计醋杆菌菌株染色体上rrn操纵子的拷贝数为四个。
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