East A K, Allaway D, Collins M D
Department of Microbiology, AFRC Institute of Food Research, Reading Laboratory, UK.
FEMS Microbiol Lett. 1992 Aug 1;74(1):57-62. doi: 10.1016/0378-1097(92)90736-8.
Amplification of the gene encoding 23S rRNA of Plesiomonas shigelloides by polymerase chain reaction (PCR), with primers complementary to conserved regions of 16S and the 3' end of 23S rRNA genes, resulted in a DNA fragment of approximately 3 kb. This fragment was cloned in Escherichia coli and its nucleotide sequence determined. The region encoding 23S rRNA shows high homology with the published sequences of 23S rRNA from other members of the gamma division of Proteobacteria. The sequence of the intergenic spacer region, between the 16S and 23S rRNA genes, was determined in a further two clones. In one the sequence of a single tRNA(Glu) was found which was absent from the other two. This variation in sequence suggests that the different clones may be derived from different ribosomal RNA operons.
使用与16S和23S rRNA基因3'端保守区域互补的引物，通过聚合酶链反应（PCR）扩增类志贺邻单胞菌23S rRNA的编码基因，得到了一个约3 kb的DNA片段。该片段被克隆到大肠杆菌中并测定了其核苷酸序列。编码23S rRNA的区域与变形菌门γ亚纲其他成员已发表的23S rRNA序列具有高度同源性。在另外两个克隆中测定了16S和23S rRNA基因之间的基因间隔区序列。在其中一个克隆中发现了单个tRNA（Glu）的序列，而另外两个克隆中没有该序列。这种序列差异表明不同的克隆可能来自不同的核糖体RNA操纵子。
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