Mekhedov S I, Kende H
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824-1312, USA.
Plant Cell Physiol. 1996 Jun;37(4):531-7. doi: 10.1093/oxfordjournals.pcp.a028976.
Partial submergence greatly stimulates internodal growth in deep water rice (Oryza sativa L.). Previous work has shown that the effect of submergence is, at least in part, mediated by ethylene, which accumulates in the air spaces of submerged internodes. To investigate the expression of the genes encoding ethylene biosynthetic enzymes during accelerated growth of deep water rice, we cloned a 1-aminocyclopropane-1-carboxylate (ACC) oxidase cDNA (OS-ACO1) from internodes of submerged plants and measured the activity of the enzyme in tissue extracts with an improved assay. We found an increase in ACC oxidase mRNA levels and enzyme activity after 4 to 24 h of submergence. Thus, it is likely that ethylene biosynthesis in internodes of deep water rice is controlled, at least in part, at the level of ACC oxidase.
部分淹没极大地刺激了深水水稻（Oryza sativa L.）节间的生长。先前的研究表明，淹没的影响至少部分是由乙烯介导的，乙烯在被淹没节间的气腔中积累。为了研究深水水稻加速生长过程中乙烯生物合成酶编码基因的表达，我们从被淹没植株的节间克隆了一个1-氨基环丙烷-1-羧酸（ACC）氧化酶cDNA（OS-ACO1），并使用改进的测定方法测量了组织提取物中该酶的活性。我们发现，淹没4至24小时后，ACC氧化酶mRNA水平和酶活性增加。因此，深水水稻节间的乙烯生物合成很可能至少部分是在ACC氧化酶水平上受到调控的。
