Ennas M G, Suzuki H, Menegazzi M, Carcereri A, Hanaoka F, Gremo F, Nieddu M, Mezzanotte R
Dipartimento di Citomorfologia, Università di Cagliari, Italy.
Heredity (Edinb). 1996 Aug;77 ( Pt 2):186-91. doi: 10.1038/hdy.1996.123.
Experiments were carried out to correlate the cytological localization of DNA polymerase alpha with the presence of its specific mRNA in human lymphocytes studied at different times after phytohaemagglutinin stimulation. Our data indicated that in resting cells it is not possible to detect DNA polymerase alpha protein or mRNA by Northern hybridization. By contrast, in stimulated cells the detection of mRNA specific for DNA polymerase alpha synthesis is possible after 16 h phytohaemagglutin stimulation, whereas immunolocalization is possible after only 4 h stimulation. Observation of cytological preparations from cells stimulated for times long enough to obtain mitoses surprisingly showed an intense immunoreaction in mitotic chromosomes treated with monoclonal antibodies to DNA polymerase alpha.
开展了多项实验，以关联DNA聚合酶α的细胞学定位及其特异性mRNA的存在情况，这些实验是在植物血凝素刺激后的不同时间点对人类淋巴细胞进行研究的。我们的数据表明，在静止细胞中，通过Northern杂交无法检测到DNA聚合酶α蛋白或mRNA。相比之下，在受刺激的细胞中，植物血凝素刺激16小时后可以检测到DNA聚合酶α合成的特异性mRNA，而免疫定位仅在刺激4小时后即可进行。对经足够长时间刺激以获得有丝分裂的细胞进行细胞学制片观察，令人惊讶地发现，在用抗DNA聚合酶α单克隆抗体处理的有丝分裂染色体中存在强烈的免疫反应。
