利用四环素阻遏物和新型四环素拮抗剂进行双向基因切换
Bi-directional gene switching with the tetracycline repressor and a novel tetracycline antagonist.
作者信息
Chrast-Balz J, Hooft van Huijsduijnen R
机构信息
Geneva Biomedical Research Institute, Geneva, Switzerland.
出版信息
Nucleic Acids Res. 1996 Aug 1;24(15):2900-4. doi: 10.1093/nar/24.15.2900.
Abstract
We have screened a panel of tetracycline (tc)-like compounds for their potential use with tc-repressor (tetR) based gene switches. The interaction between tc and tetR appears quite specific, as only tc itself and its close homologues anhydro-tc and doxycycline strongly inhibited DNA binding. However, a single tc-like compound, GR33076X, increased DNA binding of the tetR-VP16 fusion protein, both in eukaryotic cells and in bacteria. We provide evidence that this antagonist of tetracycline is potentially useful for accelerated gene switching, especially in whole animals.
摘要
我们筛选了一组四环素（tc）类化合物，以评估其与基于四环素阻遏物（tetR）的基因开关的潜在用途。四环素与tetR之间的相互作用似乎具有很高的特异性，因为只有四环素本身及其紧密同源物脱水四环素和强力霉素能强烈抑制DNA结合。然而，一种名为GR33076X的tc类化合物，在真核细胞和细菌中均能增强tetR-VP16融合蛋白与DNA的结合。我们提供的证据表明，这种四环素拮抗剂可能有助于加速基因开关的作用，尤其是在整个动物体内。
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