大肠杆菌中必需细胞分裂基因mRNA的核糖核酸酶E加工
RNase E processing of essential cell division genes mRNA in Escherichia coli.
作者信息
Cam K, Rome G, Krisch H M, Bouché J P
机构信息
Laboratoire de Microbiologie et Génétique Moleculaire, Centre National de la Recherche Scientifique, Toulouse, France.
出版信息
Nucleic Acids Res. 1996 Aug 1;24(15):3065-70. doi: 10.1093/nar/24.15.3065.
Abstract
The ratio of the FtsZ to FtsA proteins determines the correct initiation of cell division in Escherichia coli. The genes for these proteins are contiguous on the chromosome. Although both genes are transcribed from common promoters, the presence of ftsZ-specific promoters, along with differences in the efficiency of translation of their respective mRNAs, contribute to the increased relative expression of ftsZ. We report here that the polycistronic ftsA-ftsZ transcripts are cleaved by RNase E and that this cleavage affects the decay of ftsA and ftsZ mRNA. As a consequence of the cleavage, RNase E also contributes to the differential expression of the two genes.
摘要
FtsZ蛋白与FtsA蛋白的比例决定了大肠杆菌细胞分裂的正确起始。这些蛋白的基因在染色体上是相邻的。尽管这两个基因都从共同的启动子转录，但ftsZ特异性启动子的存在，以及它们各自mRNA翻译效率的差异，导致了ftsZ相对表达量的增加。我们在此报告，多顺反子ftsA-ftsZ转录本被核糖核酸酶E切割，这种切割影响ftsA和ftsZ mRNA的降解。作为切割的结果，核糖核酸酶E也导致了这两个基因的差异表达。
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