Beck B, Burlet A, Nicolas J P, Burlet C
INSERM U. 308-Mécanismes de Régulation du Comportement Alimentaire-38, NANCY, France.
Life Sci. 1996;59(4):349-56. doi: 10.1016/0024-3205(96)00304-9.
Neurotensin inhibits food intake when injected in the central nervous system and is released after fat ingestion. The aim of the present study was to measure it in different brain areas and to determine if it is involved in the long-term variations in food intake induced by the ingestion of a high-fat (HF) diet. We compared the results with those obtained with 2 low-fat [high-carbohydrates (HC)] diets and a well-balanced diet. For this purpose, weanling male Long-Evans rats were fed ad libitum for 14 weeks either on a control diet, a HF diet or a HC diet. The rats with the HC (high-starch) diet were divided into 2 subgroups: the first (HC) drank water and the second (HCS) drank a 25% sucrose solution. During the last week of the experiment, energy intake of the HCS rats was significantly greater than that of the 3 other groups of rats (+17.2%; p < 0.01; +27.1%; p < 0.001 and +34.6%; P < 0.001 vs the control, HC and HF rats respectively). NT did not vary in the midbrain and particularly in the ventral tegmental area. Its concentrations were significantly higher in the 2 HC groups than in the HF rats both in the paraventricular (PVN; p < 0.02) and dorsomedial nuclei (DMN; p < 0.03). In the DMN, they were positively correlated with energy intake (r = 0.39; p = 0.027). These results indicate that hypothalamic neurotensin is indeed involved in the long-term modulation of feeding behavior by diet composition and that fat is the more potent macronutrient for its regulation.
神经降压素注入中枢神经系统时会抑制食物摄入，且在摄入脂肪后释放。本研究的目的是测量其在不同脑区的含量，并确定它是否参与高脂（HF）饮食摄入引起的食物摄入量长期变化。我们将结果与两种低脂[高碳水化合物（HC）]饮食和一种均衡饮食的结果进行了比较。为此，将断乳雄性Long-Evans大鼠随意喂食14周，分别给予对照饮食、HF饮食或HC饮食。HC（高淀粉）饮食组的大鼠分为两个亚组：第一组（HC）饮用清水，第二组（HCS）饮用25%的蔗糖溶液。在实验的最后一周，HCS组大鼠的能量摄入量显著高于其他三组大鼠（分别比对照、HC和HF组大鼠高+17.2%；p<0.01；+27.1%；p<0.001和+34.6%；P<0.001）。中脑尤其是腹侧被盖区的神经降压素含量没有变化。在室旁核（PVN；p<0.02）和背内侧核（DMN；p<0.03）中，两个HC组的神经降压素浓度均显著高于HF组大鼠。在DMN中，它们与能量摄入量呈正相关（r=0.39；p=0.027）。这些结果表明，下丘脑神经降压素确实参与了饮食组成对摄食行为的长期调节，并且脂肪是调节其分泌的更有效的常量营养素。
