Launay T, Gallien C L, Chanoine C
Laboratoire de Biologie du Développement, URA CNRS 1188, Université René Descartes, Paris, France.
Comp Biochem Physiol B Biochem Mol Biol. 1996 Jul;114(3):257-60. doi: 10.1016/0305-0491(96)00030-2.
Myosin extracted from ventricular muscle of the urodelan amphibian Pleurodeles waltlii was analyzed in comparison with myosin extracted from skeletal muscles by native, one-dimensional SDS gel electrophoresis and two-dimensional gel electrophoresis. Two myosin isoforms were detected in ventricular muscle using pyrophosphate gel electrophoresis. These isomyosins contained two types of light chain subunits, LC1v and LC2v. Two-dimensional gel electrophoresis showed that LC1v comigrated with the slow light chain LC1s, whereas LC2v was characterized by a specific mobility, distinct from LC2s and LC2f. Diaphragm muscle was characterized by the coexistence of larval and adult myosin isoforms.
对从有尾两栖动物疣螈心室肌肉中提取的肌球蛋白，与从骨骼肌中提取的肌球蛋白进行了比较分析，采用了天然一维SDS凝胶电泳和二维凝胶电泳技术。使用焦磷酸凝胶电泳在心室肌肉中检测到两种肌球蛋白亚型。这些同工肌球蛋白包含两种轻链亚基，即LC1v和LC2v。二维凝胶电泳显示，LC1v与慢轻链LC1s迁移率相同，而LC2v具有特定的迁移率，与LC2s和LC2f不同。膈肌的特点是幼虫和成年肌球蛋白亚型共存。
