心内膜内皮细胞对血小板聚集的抑制作用。

Inhibition of platelet aggregation by endocardial endothelial cells.

作者信息

Nosaka S, Nakayama K, Hashimoto M, Sasaki T, Gu K, Saitoh Y, Kin S, Yamauchi M, Masumura S, Tamura K

机构信息

First Department of Surgery, Shimane Medical University, Japan.

出版信息

Life Sci. 1996;59(7):559-64. doi: 10.1016/0024-3205(96)00336-0.

DOI:10.1016/0024-3205(96)00336-0

PMID:8761344

Abstract

We assessed the anti-platelet properties of endocardial endothelial cells (EECs) by measuring platelet aggregation after brief interaction with EECs isolated from the right ventricles of porcine hearts. Platelet aggregation in response to thrombin was significantly inhibited by brief incubation of platelet suspensions over EEC monolayers. Pretreatment of EECs with indomethacin restored platelet reaction but that with L-NAME and hemoglobin (Hb) did not. The PGI2 content of platelet suspensions after interaction with cultured EECs was significantly correlated with the inhibition of platelet aggregation. These results suggest that EECs inhibit platelet aggregation by releasing PGI2.

摘要

我们通过测量猪心脏右心室分离出的心内膜内皮细胞（EECs）与血小板短暂相互作用后的血小板聚集情况，评估了EECs的抗血小板特性。将血小板悬浮液在EECs单层上短暂孵育后，凝血酶诱导的血小板聚集受到显著抑制。用吲哚美辛预处理EECs可恢复血小板反应，但用L-精氨酸甲酯（L-NAME）和血红蛋白（Hb）预处理则不能。与培养的EECs相互作用后，血小板悬浮液中前列环素（PGI2）的含量与血小板聚集的抑制显著相关。这些结果表明，EECs通过释放PGI2抑制血小板聚集。