一种用于检测班氏吴策线虫对波利尼西亚伊蚊感染情况的聚合酶链反应检测法。
A polymerase chain reaction assay to determine infection of Aedes polynesiensis by Wuchereria bancrofti.
作者信息
Nicolas L, Luquiaud P, Lardeux F, Mercer D R
机构信息
Institut Territorial de Recherches Médicales Louis Malarde, Papeete, Tahiti, French Polynesia.
出版信息
Trans R Soc Trop Med Hyg. 1996 Mar-Apr;90(2):136-9. doi: 10.1016/s0035-9203(96)90113-3.
The sensitivity of a previously described polymerase chain reaction (PCR) assay was improved to detect a single mosquito, infected by as few as 1-2 microfilariae of Wuchereria bancrofti, among 20-50 uninfected mosquitoes. Wild-caught Aedes polynesiensis were used to compare assessment of infection by dissection of individuals with the PCR assay of pools of mosquitoes. The PCR assay was at least as sensitive as dissection for detection of mosquitoes infected with W. bancrofti.
一种先前描述的聚合酶链反应（PCR）检测方法的灵敏度得到了提高，能够在20至50只未感染的蚊子中检测出仅感染了1至2条班氏吴策线虫微丝蚴的单个蚊子。利用野外捕获的波利尼西亚伊蚊，通过对个体进行解剖来评估感染情况，并与对蚊群进行PCR检测的结果进行比较。在检测感染班氏吴策线虫的蚊子方面，PCR检测方法的灵敏度至少与解剖法相当。
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