Cay O, Kruskal J, Thomas P, Clouse M E
Department of Radiological Sciences, Deaconess Hospital, Boston, MA 02215, USA.
J Vasc Interv Radiol. 1996 May-Jun;7(3):409-17. doi: 10.1016/s1051-0443(96)72880-4.
The distribution of different ethiodized oil-doxorubicin mixtures within and around hypovascular liver metastases in athymic nude mice was compared following either intraarterial or intraportal injections.
Six different mixtures of doxorubicin and ethiodized oil in water-soluble contrast material, shaken for 5, 10, 15, and 30 minutes, respectively, were prepared and the size of the oil droplets in the mixtures evaluated. Intraarterial and intraportal injections of mixtures shaken for 10 and 15 minutes were performed in mice bearing hypovascular hepatic colorectal metastases. In vivo video microscopy was used to evaluate microvascular flow and the biodistribution and the size of the oil droplets within and around the tumors.
Oil droplets smaller than 20 microns in diameter were taken up by tumor nodules. There was no significant difference in oil droplet accumulation within the tumor between intraarterial and intraportal injections. Oil droplets larger than 20 microns occluded small and medium portal branches. The optimum combination was 0.1 mL of ethiodized oil in 2 mL of diatrizoate sodium meglumine (Renografin-76) shaken for 15 minutes at 800 rpm, which formed droplets smaller than 20 microns (92%).
Avascular regions of hepatic metastases may be embolized with ethiodized oil-anticancer drug mixtures containing oil droplets smaller than 20 microns. Since these droplets penetrate to the tumor interstitium with either intraarterial or intraportal injections, chemoembolization via the arterial route seems preferable for treatment of unresectable hypovascular hepatic metastases since it is easier to perform. The conclusions drawn from this study are limited to the animal model for experimental hypovascular hepatic metastases.
比较在无胸腺裸鼠体内，经动脉或经门静脉注射后，不同的乙碘油-阿霉素混合物在乏血供肝转移瘤内部及周围的分布情况。
制备六种阿霉素与乙碘油在水溶性造影剂中的不同混合物，分别振荡5、10、15和30分钟，评估混合物中油滴的大小。对患有乏血供肝结肠转移瘤的小鼠进行经动脉和经门静脉注射振荡10和15分钟的混合物。使用体内视频显微镜评估微血管血流、生物分布以及肿瘤内部和周围油滴的大小。
直径小于20微米的油滴被肿瘤结节摄取。经动脉注射和经门静脉注射后，肿瘤内油滴的积聚没有显著差异。大于20微米的油滴阻塞了中小门静脉分支。最佳组合是在2毫升泛影葡胺（Renografin - 76）中加入0.1毫升乙碘油，在800转/分钟下振荡15分钟，形成小于20微米的油滴（92%）。
肝转移瘤的无血管区域可用含有小于20微米油滴的乙碘油-抗癌药物混合物进行栓塞。由于这些油滴经动脉或经门静脉注射均可渗透到肿瘤间质中，对于不可切除的乏血供肝转移瘤的治疗，经动脉途径的化疗栓塞似乎更可取，因为其操作更简便。本研究得出的结论仅限于实验性乏血供肝转移瘤的动物模型。
