Meerson F Z, Malyshev I Y, Zamotrinsky A V, Kopylov Y U
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia.
J Mol Cell Cardiol. 1996 May;28(5):835-43. doi: 10.1006/jmcc.1996.0078.
This study has shown that the maximal activation of the IP3-DAG regulatory circuit is observed on the 14th day of adaptation to repeated stresses. This activation is characterized by increased activity of phospholipase C and of the positive inotropic response of isolated heart to an alpha-agonist. Simultaneously, this activation is accompanied by the accumulation of five heat shock protein 70 (hsp70) isoforms. The IP3-DAG circuit activation and the hsp70 accumulation are accompanied by a significant increase in the cardiac resistance to post-ischemic reperfusion, as evidenced by a considerable decrease in the contracture, arrhythmias and the creatine kinase release into the perfusate. Continuation of the adaptation to repeated stresses for 28 days leads to complete reversal of the observed shifts.
本研究表明，在适应反复应激的第14天观察到IP3 - DAG调节回路的最大激活。这种激活的特征是磷脂酶C活性增加以及离体心脏对α - 激动剂的正性肌力反应增强。同时，这种激活伴随着五种热休克蛋白70（hsp70）亚型的积累。IP3 - DAG回路的激活和hsp70的积累伴随着心脏对缺血后再灌注的抵抗力显著增加，这表现为挛缩、心律失常以及灌流液中肌酸激酶释放量的显著减少。将对反复应激的适应持续28天会导致所观察到的变化完全逆转。
