Worgall S, Hänze J, Wagner R, Peiser C, Lang R E, Sulyok E, Rascher W
Department of Pediatrics, University of Giessen, Germany.
J Hypertens. 1996 May;14(5):623-8. doi: 10.1097/00004872-199605000-00012.
To characterize ouabain-like immunoreactivity in human urine.
Sensitive radioimmunoassay for ouabain characterized by high-performance liquid chromatography.
Serial dilution of urinary immunoreactive ouabain paralleled the standard curve, but not so plasma immunoreactive ouabain. Intravenous administration of 86 nmol (62.5 micrograms) ouabain caused a rapid rise in ouabain immunoreactivity in plasma of healthy volunteers with a maximum of 1.7 nmol/l 8 min after injection and returned to basal levels after 6 h. Ouabain immunoreactivity rose to 36 nmol/l in urine, suggesting that exogenously administered ouabain can be measured reliably in plasma and urine. Analytical reversed-phase high-performance liquid chromatography (isopropanol-propanol biphasic gradient; linear acetonitrile gradient) of sample extracts before assay demonstrated measurable amounts of ouabain-related material only in native urine, but not in plasma. When plasma and urine were spiked with ouabain standard or normal volunteers were injected with ouabain, the assay reliably measured ouabain.
A substance closely related to ouabain can be detected in urine, but circulates, if at all, in small amounts in human plasma.
鉴定人尿液中哇巴因样免疫反应性。
采用高效液相色谱法进行敏感的哇巴因放射免疫测定。
尿中免疫反应性哇巴因的系列稀释与标准曲线平行，但血浆中免疫反应性哇巴因则不然。静脉注射86 nmol（62.5微克）哇巴因后，健康志愿者血浆中哇巴因免疫反应性迅速升高，注射后8分钟最高达1.7 nmol/l，6小时后恢复至基础水平。尿中哇巴因免疫反应性升至36 nmol/l，表明外源性给予的哇巴因可在血浆和尿液中可靠检测。测定前对样品提取物进行反相高效液相色谱分析（异丙醇 - 丙醇双相梯度；线性乙腈梯度）显示，仅在天然尿液中可检测到一定量的哇巴因相关物质，而血浆中未检测到。当向血浆和尿液中加入哇巴因标准品或给正常志愿者注射哇巴因时，该测定法能可靠地检测哇巴因。
在尿液中可检测到一种与哇巴因密切相关的物质，但在人血浆中即使存在也仅以少量形式循环。
