甲醛存在下用单扫描示波极谱法测定阿米卡星

[Determination of amikacin by single oscillopolarography in the presence of formaldehyde].

作者信息

Sun C L, Mo W M, Wang J X, Chen Y H, Hu Z M

机构信息

Department of Chemical Engineering, Zhejiang University of Technology, Hangzhou.

出版信息

Yao Xue Xue Bao. 1996;31(2):126-31.

PMID:8762471

Abstract

The singlescan oscillopolarographic behavior of formaldehyde derivative of amikacin (AMK) in the medium of Britton-Robinson buffer solution (pH 8.0) has been studied and the mechanism of its electrode process has been discussed. Under optimum conditions, the peak current increases linearly with the increase of AMK concentration in the range of 6.0 x 10(-7)-5.0 x 10(-5) mol.L-1 and its correlation coefficient is 0.9988. The detection limit is 2.4 x 10(-7) mol.L-1. This method has been applied to the determination of trace AMK in samples of its injection, urine and blood serum without pretreatment. Its recovery was 90%-100% and the relative standard deviation was less than 4.1%.

摘要

研究了丁胺卡那霉素（AMK）甲醛衍生物在Britton-Robinson缓冲溶液（pH 8.0）介质中的单扫描示波极谱行为，并探讨了其电极过程机理。在最佳条件下，峰电流在6.0×10⁻⁷ - 5.0×10⁻⁵ mol·L⁻¹范围内随AMK浓度的增加呈线性增加，其相关系数为0.9988。检测限为2.4×10⁻⁷ mol·L⁻¹。该方法无需预处理即可用于测定其注射液、尿液和血清样品中的痕量AMK。其回收率为90% - 100%，相对标准偏差小于4.1%。