Li D, Chen W, Zhou H
Tangdu Hospital, Fourth Medical University, Xian.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1995;30(5):299-301.
The laryngeal epithelial cells for culture came from healthy fetuses of induced labor of middle and late period. Cells were cultured under 37 degrees C, 5% carbon dioxide and saturated humidity. The results showed that in the primary generation was cultured for 7 days, the aggregens could be seen by the naked eye. In 10-14 days, the cell layers formed and gradually covered the whole bottom of the flasks. The second generation grew faster than the primary one. Twenty flasks of the cultured cells were freeze-preserved for 14 to 28 days, of which 5 flasks (25%) were resuscitated successfully. By means of the cell culture, some characteristics of the laryngeal epithelial cells can be studied.
用于培养的喉上皮细胞来自中期和晚期引产的健康胎儿。细胞在37摄氏度、5%二氧化碳和饱和湿度条件下培养。结果显示，原代培养7天时，肉眼可见细胞聚集体。10至14天时，细胞层形成并逐渐覆盖整个培养瓶底部。第二代细胞生长比原代快。20瓶培养细胞冻存14至28天，其中5瓶（25%）复苏成功。通过细胞培养，可以研究喉上皮细胞的一些特性。
