Seo H, Xie B, Wang S, Yoshikawa H, Oyamada T, Yoshikawa T
Department of Veterinary Pathology, School of Medicine and Animal Sciences, Kitasato University, Japan.
J Comp Pathol. 1996 Apr;114(3):283-90. doi: 10.1016/s0021-9975(96)80050-8.
The livers of 13 Sika deer (Cervus nippon Temminck) aged 4 to 9 years and suffering from copper deficiency (enzootic ataxia) were examined histologically, histochemically and by electron microscopy. In addition, the serum and liver copper concentrations, measured in three animals, were found to be low. Histologically, the hepatocytes exhibited cloudy swelling, and numerous haemosiderin deposits were seen in the hepatocytes and Kupffer cells. Staining with p-dimethyl amino-benzylidene-rhodamine revealed distinctly fewer copper granules than normal. Histochemically, 3,3'-diaminobenzidine-H2O2 staining revealed increased numbers of catalase-positive granules around nuclei. Electron microscopically, "giant" and bizarre-shaped mitochondria, irregular depression of the mitochondrial membrane, and fusion of cristae were noted. Disorders of copper-containing enzymes, including cytochrome oxidase, caeruloplasmin and monoamine oxidase, may have been responsible for the mitochondrial abnormalities.
对13只4至9岁患有铜缺乏症（地方流行性共济失调）的梅花鹿（Cervus nippon Temminck）的肝脏进行了组织学、组织化学和电子显微镜检查。此外，在三只动物中测得的血清和肝脏铜浓度较低。组织学上，肝细胞出现浊肿，在肝细胞和枯否细胞中可见大量含铁血黄素沉积。用对二甲氨基苄叉罗丹明染色显示铜颗粒明显少于正常情况。组织化学上，3,3'-二氨基联苯胺-H2O2染色显示细胞核周围过氧化氢酶阳性颗粒数量增加。电子显微镜下，可见“巨大”和形状怪异的线粒体、线粒体膜的不规则凹陷以及嵴的融合。包括细胞色素氧化酶、铜蓝蛋白和单胺氧化酶在内的含铜酶紊乱可能是线粒体异常的原因。
