在以铜绿假单胞菌外膜蛋白OprF为载体的表位呈递系统中，疟原虫表位长度对其抗原性和免疫原性的影响。
The effect of the length of a malarial epitope on its antigenicity and immunogenicity in an epitope presentation system using the Pseudomonas aeruginosa outer membrane protein OprF as the carrier.
作者信息
Wong R S, Hancock R E
机构信息
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
出版信息
FEMS Microbiol Lett. 1996 Jul 1;140(2-3):209-14. doi: 10.1016/0378-1097(96)00182-6.
This study showed that the antigenicity of a malarial epitope increased with the length of the epitope when inserted at positions aa26 (amino acid position 26) and aa196, but not at aa213, of the Pseudomonas aeruginosa major outer membrane protein OprF (326 amino acids). Immunization studies showed that a 19-aa epitope was significantly more immunogenic than a 7-aa epitope when inserted at aa26 of OprF, while neither an 11- nor a 19-aa epitope fused to the C-terminus of glutathione S-transferase was immunogenic.
本研究表明，当插入铜绿假单胞菌主要外膜蛋白OprF（326个氨基酸）的第26位氨基酸（aa26）和第196位氨基酸处时，疟疾表位的抗原性随表位长度增加而增强，但插入第213位氨基酸处时则不然。免疫研究表明，当插入OprF的aa26处时，一个19个氨基酸的表位比一个7个氨基酸的表位免疫原性显著更强，而与谷胱甘肽S-转移酶C末端融合的11个氨基酸或19个氨基酸的表位均无免疫原性。
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