Mandelbrot L, Brossard Y, Aubin J T, Bignozzi C, Krivine A, Simon F, Dommergues M
Department of Obstetrics and Gynecology, Hôpital Cochin-Port Royal, Paris, France.
Am J Obstet Gynecol. 1996 Aug;175(2):489-93. doi: 10.1016/s0002-9378(96)70167-9.
Our purpose was to evaluate the potential of midtrimester fetal blood sampling to detect in utero human immunodeficiency virus type 1 infection.
Ultrasonographically guided fetal blood sampling was performed before pregnancy termination in 28 women infected with human immunodeficiency virus type 1. Mean gestational age was 22 weeks (range 15 to 29 weeks). Samples were tested for human immunodeficiency virus with undissociated p24 antigen and deoxyribonucleic acid polymerase chain reaction or viral isolation by cell culture. After terminations fetal thymuses were also tested for human immunodeficiency virus infection by polymerase chain reaction. Polymerase chain reaction was also performed on maternal cells to confirm that the primer pairs used were able to detect human immunodeficiency virus type 1 strains present.
All fetal blood samples had negative results by p24 antigen and polymerase chain reaction or virus culture and all fetal thymuses were negative by polymerase chain reaction.
Early in utero human immunodeficiency virus infection appears infrequent, supporting the hypothesis that mother-to-child transmission predominantly occurs late in pregnancy. Therefore midtrimester prenatal diagnosis is not currently of use to women in making reproductive decisions.
我们的目的是评估孕中期胎儿采血检测子宫内人类免疫缺陷病毒1型感染的可能性。
对28例感染人类免疫缺陷病毒1型的孕妇在终止妊娠前进行超声引导下的胎儿采血。平均孕周为22周（范围15至29周）。采用未分离的p24抗原、脱氧核糖核酸聚合酶链反应或细胞培养病毒分离法对样本进行人类免疫缺陷病毒检测。终止妊娠后，也通过聚合酶链反应检测胎儿胸腺是否感染人类免疫缺陷病毒。对母体细胞也进行聚合酶链反应，以确认所用引物对能够检测出存在的人类免疫缺陷病毒1型毒株。
所有胎儿血样的p24抗原、聚合酶链反应或病毒培养结果均为阴性，所有胎儿胸腺的聚合酶链反应结果均为阴性。
子宫内早期人类免疫缺陷病毒感染似乎很少见，这支持了母婴传播主要发生在妊娠晚期的假说。因此，目前孕中期产前诊断对女性做出生殖决策并无帮助。
