Banger K K, Lock E A, Reed C J
School of Biomolecular Sciences, Liverpool John Moores University, Liverpool, U.K.
Biochem Pharmacol. 1996 Sep 13;52(5):801-8. doi: 10.1016/0006-2952(96)00341-3.
The glutathione S-transferases (GSTs) of rat olfactory epithelium have been characterised with regard to sex differences, induction, and developmental regulation, and compared to those of the liver. Olfactory cytosolic GST activity with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate was similar in both male and female animals, and there were no differences in subunit profile. Administration of trans-stilbene oxide and beta-naphthoflavone had no effect on olfactory GST activity with CDNB, although phenobarbitone treatment resulted in a small, but significant, increase in activity (130% compared to controls). HPLC analysis of subunit profiles indicated that all three agents induced olfactory subunit 1b and decreased subunit 6. The effect of age (3 to 84 days) on both cytosolic and microsomal CDNB activity was examined. In the liver, cytosolic activity was low at 3 days and climbed steadily to reach maximal levels around 28 days, but microsomal activity was relatively constant at all ages. Olfactory cytosolic activity was similar at all ages; microsomal activity was low until 21 days and then increased to reach a maximum at 56 days. Changes in individual cytosolic subunits were assessed by SDS-PAGE followed by immunoblotting. The significance of these results with regard to putative physiological roles for olfactory GSTs is discussed.
已对大鼠嗅觉上皮中的谷胱甘肽S-转移酶（GSTs）的性别差异、诱导作用及发育调控进行了表征，并与肝脏中的谷胱甘肽S-转移酶进行了比较。以1-氯-2,4-二硝基苯（CDNB）为底物时，雄性和雌性动物嗅觉胞质GST活性相似，亚基谱也无差异。给予反式氧化茋和β-萘黄酮对以CDNB为底物的嗅觉GST活性无影响，尽管苯巴比妥处理导致活性有小幅但显著的增加（与对照组相比增加了130%）。亚基谱的HPLC分析表明，所有三种试剂均诱导了嗅觉亚基1b并降低了亚基6。研究了年龄（3至84天）对胞质和微粒体CDNB活性的影响。在肝脏中，胞质活性在3天时较低，随后稳步上升，在约28天时达到最高水平，但微粒体活性在所有年龄相对恒定。嗅觉胞质活性在所有年龄相似；微粒体活性在21天前较低，然后增加，在56天时达到最大值。通过SDS-PAGE随后进行免疫印迹评估单个胞质亚基的变化。讨论了这些结果对于嗅觉GSTs假定生理作用的意义。
